Low endotoxin fbs

RPMI 1640 and low-endotoxin FBS were obtained from Lonza (Walkersville, MD, USA). Recombinant human GM-CSF and human IL-4 were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany); Flow cytometric analysis was performed using the following mouse anti-human antibodies: CD83 (HB15e) and CD1a (HI149) (Becton Dickinson, San Jose, CA, USA); CD80 (2D10), CD86 (T2.2), HLA-DR (L243) and CD14 (M5E2) (Biolegend, San Diego, CA, USA). PLGA (poly[DL-lactide-co-glycolide] 50:50 lactide-glycolide ratio, CAS 26780-50-7), PVA (poly[vinyl alcohol], CAS 9002-89-5), Acetone (≥99% purity, 1.00013), Dimethyl sulfoxide (DMSO, ≥99% purity D-5879), Oxyresveratrol (≥97% purity, 91211) were purchased from Sigma-Aldrich, (St. Louis, MO, USA).

RPMI 1640 and low-endotoxin FBS were obtained from Lonza (Walkersville, MD, USA); flow cytometric analysis was performed using mouse anti-human antibody CD14 (M5E2) (Biolegend, San Diego, CA, USA).
PLGA (poly[DL-lactide-co-glycolide], CAS 26780-50-7), PVA (poly[vinyl alcohol], CAS 9002-89-5), acetone (1.00013), dimethyl sulfoxide (DMSO, D-5879), oxyresveratrol (91211) and β-glucan from baker’s yeast were purchased from Sigma-Aldrich (St. Louis, MO, USA).