Anti ubiquitin 3936 s

The following reagents were used in this study: lipofectamine 2000 (11668019; Invitrogen), polybrene (TR-1003; Sigma-Aldrich), actinomycin D (S8964; Selleck), DAPI (H1200; Vector Laboratories), complete EDTA-free protease inhibitor cocktail (K1007; APExBIO), protein A/G agarose beads (20421; Thermo Fisher Scientific), anti-Flag M2 affinity agarose beads (A2220; Sigma), and streptavidin agarose resin (20349; Thermo Fisher Scientific).
The following antibodies were used in this study: anti-HK2 (22029-1-AP), anti-GPI (15171-1-AP), anti-ALDOB (18065-1-AP), anti-PGK1 (17811-1-AP), anti-PGAM1 (16126-1-AP), anti-ENO1 (11204-1-AP), anti-LDHA (19987-1-AP), and PABPC1 (10970-1-AP) antibodies were purchased from Proteintech. Anti-GFP (sc-9996), anti-β-actin (sc-47778), and anti-GAPDH (sc-166545) antibodies were purchased from Santa Cruz Biotechnology. Anti-c-Myc (9402 S), anti-PKM2 (4053 S), and anti-ubiquitin (3936 S) antibodies were purchased from Cell Signaling Technology. Anti-PFKL (ab181064) antibody was purchased from Abcam. Anti-Flag (F3165) and anti-MKRN3 (HPA029494) antibodies were purchased from Sigma-Aldrich. Horseradish peroxidase-conjugated secondary antibodies against rabbit (111-035-144) and mouse (115-035-062) were purchased from Jackson ImmunoResearch.

MTX-211 (S6541) was purchased from Selleck. L-Glutathione reduced (GSH) (HY-D0187) was obtained from MedChem Express (Princeton, NJ, USA). The antibodies used in the following experiments were anti-PARP (9532S, 1:1000), anti-caspase3 (9662S, 1:1000), anti-p21 (2947S, 1:1000), and anti-ubiquitin (3936S, 1:1000) obtained from Cell Signaling Technology; anti-keap1 (sc-365626, 1:1000), anti-GCLM (sc-55586, 1:1000) were from Santa Cruz; and anti-NRF2 (T55136, Abmart, 1:1000), anti-keap1 (10503-2-AP, Proteintech, 1:2000), and anti-p62 (ab109012, Abcam, 1:10,000). Anti-rabbit IgG (DyLight 488, FD0136) and anti-mouse IgG (DyLight 649, FD0144) were from Hangzhou Fude biological technology (Fdbio science, Hangzhou, China).