Thy1 yfp 16

Manufactured by Jackson ImmunoResearch

Sourced in United States

Thy1-YFP-16 is a fluorescently-labeled antibody produced by Jackson ImmunoResearch. It is targeted against the Thy1 antigen and is conjugated with the Yellow Fluorescent Protein (YFP) reporter. This product can be used for various immunohistochemical and flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Thy1-YFP (16 citations) B6.Cg-Tg (Thy1-YFP) 16Jrs/J (Thy1-YFP-16) (2 citations)

5 protocols using thy1 yfp 16

Genetic Manipulation of Murine Models

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were purchased from the Jackson Lab with genotypes including C57BL/6 (JAX # 000664), Thy1-EGFP-M (JAX# 007788), Thy1-YFP-16 (JAX #003709), Ai14 (JAX# 007908), Ai 140 (JAX# 030220), Shh-Cre^ERT2 (JAX# 005623) and Gli1-cre^ERT2 (JAX# 007913). All animal experiments were approved by the Institutional Animal Care and Use Committee of the Chinese Institute for Brain Research.
For tamoxifen treatment, tamoxifen (Sigma-Aldrich, T5648) was dissolved in corn oil (Sigma-Aldrich, C8267) at 20 mg/mL. The solution was kept at –20 °C and delivered via intraperitoneal injection or oral gavage for postnatal treatments.

Yi Y., Li Y., Zhang S., Men Y., Wang Y., Jing D., Ding J., Zhu Q., Chen Z., Chen X., Li J.L., Wang Y., Wang J., Peng H., Zhang L., Luo W., Feng J.Q., He Y., Ge W.P, & Zhao H. (2024). Mapping of individual sensory nerve axons from digits to spinal cord with the transparent embedding solvent system. Cell Research, 34(2), 124-139.

+ Open protocol

+ Expand

Transgenic Mouse Strains for Neuroscience

Check if the same lab product or an alternative is used in the 5 most similar protocols

All mice were treated in accordance with guidelines of the Uniformed Services University of the Health Sciences (USUHS) and the National Institutes of Health Guide for the Care and Use of Laboratory Animals. Mice were socially housed as 2–4 mice per cage in 35 cm × 16.5 cm × 18 cm cages that contained enrichment objects and maintained a 12-h daytime light cycle (0600–1800) during which all experimental procedures were performed. The following mouse strains were obtained from the Jackson Laboratory (Bar Harbor, ME, USA): NG2CreER (RRID: IMSR_JAX:008538; B6.Cg-Tg(Cspg4-cre/Esr1*)BAkik/J">B6.Cg-Tg(Cspg4-cre/Esr1*)BAkik/J), ROSA^mT/mG (RRID:IMSR_JAX:007676; B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J), and Thy1-YFP-16 (RRID:IMSR_JAX:003709; B6.Cg-Tg(Thy1-YFP)16Jrs/J">B6.Cg-Tg(Thy1-YFP)16Jrs/J). Mouse strains were bred as in-house colonies to generate experimental mice. First-generation offspring from NG2CreER and ROSA^mTmG breeders were used as NG2CreER;mTmG mice. The total number of Thy1-YFP-16 mice used was 15 (8 male, 7 female) and the total number of NG2CreER;mTmG mice used was 14 (10 male, 4 female). Male and female mice were combined for analyses after separate analyses showed no sex-based differences. Littermates of Thy1-YFP-16 mice or NG2CreER;mTmG mice were used for quantitative analyses. The number of mice for each condition in each experiment is provided in each figure legend.

Bradshaw DV J.r., Kim Y., Fu A., Marion C.M., Radomski K.L., McCabe J.T, & Armstrong R.C. (2021). Repetitive Blast Exposure Produces White Matter Axon Damage without Subsequent Myelin Remodeling: In Vivo Analysis of Brain Injury Using Fluorescent Reporter Mice. Neurotrauma Reports, 2(1), 180-192.

+ Open protocol

+ Expand

Genetically Modified Mouse Strains for Neuroscience

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were housed in a temperature‐ and humidity‐controlled vivarium on a 12 h–12 h light–dark cycle with free access to food and water.
The following strains were used: Asic2 KO (Asic2^tm1Wsh/J; The Jackson Laboratory; #013126), Thy1‐YFP16 (The Jackson Laboratory; #003709), Pvalb^Cre (Pvalb^tm1(cre)Arbr/J; The Jackson Laboratory; #017320) and Rosa26^tdTomato (The Jackson Laboratory, #007909). In all experiments, Asic2 KO mice are Asic2^−/− and control mice are littermates that are either Asic2^+/+ or Asic2^−/+. Mice were genotyped by PCR of genomic DNA from ear clips. Primer sequences and amplicon sizes are listed in Table 1.

Bornstein B., Watkins B., Passini F.S., Blecher R., Assaraf E., Sui X.M., Brumfeld V., Tsoory M., Kröger S, & Zelzer E. (2023). The mechanosensitive ion channel ASIC2 mediates both proprioceptive sensing and spinal alignment. Experimental Physiology, 109(1), 135-147.

+ Open protocol

+ Expand

Genetic Mouse Models for Mechanosensation

Check if the same lab product or an alternative is used in the 5 most similar protocols

All experiments involving mice were approved by the Institutional Animal Care and Use Committee (IACUC) of the Weizmann Institute. Mice were housed in a temperature- and humidity-controlled vivarium on a 12 hr light-dark cycle with free access to food and water.
The following strains were used: Piezo2^{EGFP-IRES-Cre} (The Jackson Laboratory, #027719), Gli1^CreERT2 (The Jackson Laboratory, #007913), Rosa26^tdTomato (The Jackson Laboratory, #007909), and Thy1-YFP16 (The Jackson Laboratory, #003709).
In all experiments, at least three mice from different litters were used. Mice were genotyped by PCR of genomic DNA from ear clips. Primer sequences and amplicon sizes are listed in Table 1.

Bornstein B., Heinemann-Yerushalmi L., Krief S., Adler R., Dassa B., Leshkowitz D., Kim M., Bewick G., Banks R.W, & Zelzer E. (2023). Molecular characterization of the intact mouse muscle spindle using a multi-omics approach. eLife, 12, e81843.

+ Open protocol

+ Expand

Mice Aging and TBI Model Study

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

At the start of experiments, mice were 8–10 weeks old, which approximately corresponds to young adulthood in humans [26 (link)], a patient population at high risk of TBI [73 (link)]. Transgenic mice expressing YFP under the control of the Thy-1 promoter (Thy1-YFP-16) on a C57BL/6 background were acquired from Jackson Laboratories (Bar Harbor, ME). The original Thy1-YFP-16 mice (IMSR Cat# JAX:003,709, RRID:IMSR_JAX:003,709; B6.Cg-Tg(Thy1-YFP)16Jrs/J) were bred in-house to generate the experimental male (n = 3/group) and female (n = 4/group) mice for Study 1 – Confocal Analysis. As no sex-based differences were detected in this initial study, the experiments of Studies 2 and 3 used only male mice. C57BL/6J (IMSR Cat# JAX:000,664, RRID:IMSR_JAX:000,664) mice were purchased from Jackson Laboratories. Mice were socially housed in 35 cm × 16.5 cm × 18 cm enrichment cages (2–5 mice per cage), exposed to 12:12 h light–dark cycle, at controlled room temperature, and with free access to food and water. All experimental procedures were conducted during the daytime light cycle (0600–1800) after the mice had acclimated for at least 3 days in their home cage.

Radomski K.L., Zi X., Lischka F.W., Noble M.D., Galdzicki Z, & Armstrong R.C. (2022). Acute axon damage and demyelination are mitigated by 4-aminopyridine (4-AP) therapy after experimental traumatic brain injury. Acta Neuropathologica Communications, 10, 67.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!