The morphology of the LnNPs was analyzed on a JEM-2100F (JEOL Ltd., Japan) installed at Hanyang LINC3.0 Analytical Equipment Center (Hanyang University, Seoul, Republic of Korea) at an accelerating voltage of 200 kV. The XRD patterns of the LnNPs were characterized by an XRD-7000 diffractometer. The Fourier transform infrared (FT-IR) spectra of the LnNPs were obtained by using a Nicolet iS50 FT-IR spectrophotometer (Thermo Fisher Scientific Co., USA). The hydrodynamic diameter and zeta potential were measured by a Zetasizer Nano ZSP (Malvern Co., UK). The photoluminescence (PL) emission spectra were recorded by a spectrometer (Andor, Kymera 193i) and an intensified sCMOS detector (Andor, ISTAR-SCMOS-18F-73) under external excitation at 980 nm provided by a 980 nm laser diode (Changchun New Industries Optoelectronics Tech. CO., China, MDL-III-980-2W). The PL lifetime was measured using a photomultiplier tube detector (H10721-01; Hamamatsu, Shizuoka, Japan) attached to the spectrometer and a digital oscilloscope (Rhode & Schwarz, Munich, Germany, RTM3002) under excitation at 980 nm using pulsed laser (optical parametric oscillator (OPO) laser, Q-switched Nd-YAG laser, EKSPLA NT342). The PL emission wavelength was selectively measured using a bandpass filter (Semrock, ff-01-800/12-25) and shortpass filter (Semrock, ff-01-950/sp-25) placed in front of the detectors.
Kymera 193i
Manufactured by Oxford Instruments
The Kymera 193i is a high-performance imaging spectrograph designed for a variety of applications. It features a focal plane array detector and a versatile optical design that enables the acquisition of spectral information.
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Lab products found in correlation
4 protocols using kymera 193i
1
Characterization of Lanthanide Nanoparticles
2
Polarized Scattering Characterization of GNR-Microfiber System
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Scattering spectra of the coupled system were collected using a dark-field setup as shown in fig. S1. We used a beam of unpolarized white light (SC-5, Wuhan Yangtze Soton Laser Co. Ltd.) from a photonic crystal fiber to illuminate microfiber-coupled GNRs at an oblique angle of about 30° with respect to the microfiber axis. We used a 100× objective (NA = 0.7; CFI L plan BD ELWD, Nikon) to collect the scattered light, which was then redirected to a spectrometer (Kymera 193i, Andor) for spectral analysis and a CCD camera (DS-Fi3, Nikon) for imaging. A linear polarizer was inserted before the spectrometer and CCD camera to study the polarization property.
For the location of single GNRs (as well as the determination of GNRs’ orientation) on active microfibers, a 640-nm laser (away from the absorption spectral range of the doped dye) was used as the illumination light source. GNRs with the orientation of their long axes perpendicular to the microfiber length (checked with a polarizer) were chosen for the following optical lasing characterization.
For the location of single GNRs (as well as the determination of GNRs’ orientation) on active microfibers, a 640-nm laser (away from the absorption spectral range of the doped dye) was used as the illumination light source. GNRs with the orientation of their long axes perpendicular to the microfiber length (checked with a polarizer) were chosen for the following optical lasing characterization.
3
Imaging Unroofed Cells with α-Synuclein
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Unroofed cells were incubated with NBD-labeled α-syn for 5 min at room temperature, washed with stabilization buffer three times, and immediately after fixed in 2% paraformaldehyde for 20 min and stored at 4 °C. Unroofed cells were imaged using a 100× silicon oil-immersion objective (Olympus; UPLSAPO100XS) on an inverted microscope (Olympus; IX-73) equipped with an imaging spectrograph (Kymera 193i; Andor Oxford Instruments) with a 600 g/mm grating. The 488-nm line of an Ar-ion laser (Modu-Laser) was pass through a laser clean-up filter and directed to the sample using a 488-nm dichroic. To remove residual excitation light, a 488-nm long-passed emission filter was used. Each image and spectrum was acquired using 5-s exposure time and 10 accumulations with a slit width of 500 µm.
4
Ultrafast Transient Absorption Spectroscopy
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Pump/probe measurements were performed with a Yb:KGW (ytterbium-doped with potassium, gadolinium and tungstate fiber laser) fs laser, characterized by ~ 200 fs pulse duration and centered at 1030 nm (PHAROS: Light Conversion). A simplified picture of the experimental setup is displayed in Fig. S1 , while a more detailed description is reported in25 (link). The tunable excitation wavelength is generated through an OPA (optical parametric amplifier), from Light Conversion, delivering pulses at a repetition rate of 60 kHz. Samples were excited with femtosecond pulses in the UV range, at fixed energy per pulse of ~ 50 nJ/pulse, from 320 nm (3.9 eV) to 230 nm (5.4 eV), following the transient absorption resonantly with the 5.1 eV absorption band. The pump and the probe spots dimensions focalized into the samples have a diameter of ~ 100 µm and ~ 90 µm, respectively. The optical delay line allows us to control the delay between the two beams up to a maximum of ~ 7 ns. The transient absorption response, chopped at 75 Hz, was probed in the UV/visible (370 ÷ 650 nm) spectral range. The transmitted probe light is acquired using an imaging spectrograph (Kymera 193i, OXFORD instruments) equipped with a NMOS linear image sensor (HAMAMATSU S8380-256Q). Every spectrum is the average of 4000 spectra performed in the whole investigated window under these experimental conditions.
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