LC–Q–TOF–MS analysis was carried out using an Agilent Technologies (Santa Clara, CA, USA) 1290 Infinity Series liquid chromatograph coupled with an Agilent Technologies 6500 iFunnel Q–TOF LC/MS device equipped with an electrospray ionization Agilent Technologies Jet Stream ion source. Chromatographic separation was achieved on an Agilent ZORBZAX 300 SB-C18 column (4.6 mm × 250 mm) (Agilent, Santa Clara, CA, USA). Injection volume was 20 µL. The mobile phase consisted of 0.1% formic acid in Milli-Q water (solvent A) and 0.1% formic acid in 100% acetonitrile (solvent B) at a flow rate of 0.5 mL/min. The mobile-phase gradient (10–60% B) was applied. Source nitrogen gas temperature was 325 °C, sheath gas flow was 12 L/min, and nebulizer pressure was 40 psig. Voltages were set at 4000 (capillary) and 175 V (fragmentor). Positive ions were acquired in the range of 100–3200 m/z for MS scans. Internal mass correction was enabled by using two reference masses at 121.0509 and 922.0098 m/z. Data acquisition and instrument control were performed using Agilent MassHunter Workstation software (B.06.01 SPI).
1290 infinity series liquid chromatograph
Manufactured by Agilent Technologies
Sourced in United States, Germany
The 1290 Infinity Series liquid chromatograph is a high-performance liquid chromatography (HPLC) instrument designed for analytical and preparative applications. It features advanced technology to provide high-resolution separation, accurate quantification, and reliable performance.
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2 protocols using 1290 infinity series liquid chromatograph
1
Liquid Chromatography-Mass Spectrometry Analysis
2
Quantitative Metabolite Analysis via LC-MS
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The equipment used for sample extraction and purification included a SORVALL ST 16R centrifuge (Thermo), a rotary evaporator (RV 10 Basic, IKA, China), a nitrogen concentration instrument (ANPEL, China) and ultrapure water production equipment (Millipore, USA). LC-ESI-MS/MS analysis was performed using a 1290 Infinity series liquid chromatograph (Agilent Technologies, Germany) with an ACQUITY UPLC CSH C18 HPLC column (2.1 × 100 mm; 1.7-µm particle size) (Waters, USA) and a 6470 Triple Quad series mass spectrometer (Agilent).
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