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P 1000 instrument

Manufactured by Sutter Instruments
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Sourced in Germany

The P-1000 is a micropipette puller instrument designed for laboratory use. It is used to create glass micropipettes from glass capillary tubes through a heating and pulling process.

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Lab products found in correlation

Biocytin, by Merck Group (2 mentions)

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P-1000 (100 citations)

2 protocols using p 1000 instrument

1

Whole-cell Recordings of Neuronal Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole-cell recordings were carried out using borosilicate glass
electrodes (Hilgenberg, Germany) of 3-4 MΩ pulled on a P-1000 instrument
(Sutter). Electrodes were filled with an intracellular solution containing (in
mM): 130 K-gluconate, 3 KCl, 4 ATP-Na2, 0.35 GTP-Na2, 8
phosphocreatine-Na2, 10 HEPES, 0.5
ethyleneglycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetate
(EGTA), (pH 7.2 set with KOH) and 0.5% biocytin (Sigma) for
post-hoc cell identification. After recordings, brain
slices were immersion-fixed in 4% PFA at 4°C overnight. Recordings were
carried out on an EPC-10 triple amplifier (HEKA) controlled by PatchMaster
2.80.
Romanov R.A., Tretiakov E.O., Kastriti M.E., Zupancic M., Häring M., Korchynska S., Popadin K., Benevento M., Rebernik P., Lallemend F., Nishimori K., Clotman F., Andrews W.D., Parnavelas J.G., Farlik M., Bock C., Adameyko I., Hökfelt T., Keimpema E, & Harkany T. (2020). Molecular design of hypothalamus development. Nature, 582(7811), 246-252.
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2

Whole-cell Recordings of Neuronal Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole-cell recordings were carried out using borosilicate glass
electrodes (Hilgenberg, Germany) of 3-4 MΩ pulled on a P-1000 instrument
(Sutter). Electrodes were filled with an intracellular solution containing (in
mM): 130 K-gluconate, 3 KCl, 4 ATP-Na2, 0.35 GTP-Na2, 8
phosphocreatine-Na2, 10 HEPES, 0.5
ethyleneglycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetate
(EGTA), (pH 7.2 set with KOH) and 0.5% biocytin (Sigma) for
post-hoc cell identification. After recordings, brain
slices were immersion-fixed in 4% PFA at 4°C overnight. Recordings were
carried out on an EPC-10 triple amplifier (HEKA) controlled by PatchMaster
2.80.
Romanov R.A., Tretiakov E.O., Kastriti M.E., Zupancic M., Häring M., Korchynska S., Popadin K., Benevento M., Rebernik P., Lallemend F., Nishimori K., Clotman F., Andrews W.D., Parnavelas J.G., Farlik M., Bock C., Adameyko I., Hökfelt T., Keimpema E, & Harkany T. (2020). Molecular design of hypothalamus development. Nature, 582(7811), 246-252.
+ Open protocol
+ Expand

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