Yohimbine hydrochloride

Manufactured by Bio-Techne

Sourced in United Kingdom

Yohimbine hydrochloride is a chemical compound used in laboratory research. It is a naturally occurring indole alkaloid derived from the bark of the Pausinystalia yohimbe tree. Yohimbine hydrochloride is commonly used as a research tool in scientific studies, but its specific applications and intended uses are not provided.

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Lab products found in correlation

Prazosin hydrochloride, by Merck Group (3 mentions) Picrotoxin ptx, by Bio-Techne (2 mentions) L norepinephrine bitartrate salt monohydrate ne, by Merck Group (2 mentions) Tetrodotoxin ttx, by Bio-Techne (2 mentions) R phenylephrine hydrochloride, by Merck Group (2 mentions) Tnp atp triethylammonium salt, by Bio-Techne (2 mentions) Pyridoxalphosphate 6 azophenyl 2 4 disulfonic acid tetrasodium salt ppads, by Bio-Techne (2 mentions) Clonidine hydrochloride, by Bio-Techne (2 mentions) Rx821002, by Bio-Techne (2 mentions) Dopamine hydrochloride da, by Merck Group (1 mentions) Haloperidol hydrochloride, by Bio-Techne (1 mentions) R sch 23390 hydrochloride, by Merck Group (1 mentions) Acetylcholine chloride, by Merck Group (1 mentions) Histamine, by Nacalai Tesque (1 mentions) Skf81297 hydrobromide, by Bio-Techne (1 mentions) Pertussis toxin, by Merck Group (1 mentions) Dopamine hydrochloride, by Merck Group (1 mentions) Quinpirole hydrochloride, by Bio-Techne (1 mentions) 3h sch23390, by PerkinElmer (1 mentions) Ro 105824 dihydrochloride, by Bio-Techne (1 mentions)

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Yohimbine (16 citations)

12 protocols using yohimbine hydrochloride

Pharmacological Manipulation of Neurochemicals

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The following drugs were kept at −20°C as stock solutions and dissolved in aCSF to their final concentrations on the day of experiments: L-(−)-norepinephrine (+)-bitartrate salt monohydrate (NE, 10-100 μM, Sigma-Aldrich, shielded from light exposure during preparation, storage and application), picrotoxin (PTX, 50 μM, Tocris), prazosin hydrochloride (10 μM, Sigma-Aldrich), (R)-(−)-phenylephrine hydrochloride (100 μM, Sigma-Aldrich), yohimbine hydrochloride (20 μM, Tocris), tetrodotoxin (TTX, 1 μM, Tocris), TNP-ATP triethylammonium salt (10 μM, Tocris), pyridoxalphosphate-6-azophenyl-2′,4’-disulfonic acid tetrasodium salt (PPADS, 100 μM, Tocris).
Fluorocitric acid (FCA, Sigma-Aldrich) was prepared on the day of experiments with DL-fluorocitric acid barium salt (Paulsen et al., 1987 (link)) dissolved in 1 mL of 0.1 M HCl. Two-to-three drops of 0.1 M Na₂SO₄ was added to precipitate the Ba²⁺, after which 2 mL of 0.1 M Na₂HPO₄ was added and the suspension was centrifuged at 1000 g for 5 min. The supernatant was added to the aCSF to achieve a concentration of 100 μM.

Chen C., Jiang Z., Fu X., Yu D., Huang H, & Tasker J.G. (2019). Astrocytes Amplify Neuronal Dendritic Volume Transmission Stimulated by Norepinephrine. Cell reports, 29(13), 4349-4361.e4.

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Neurotransmitter Stock Solution Preparation

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Dopamine (DA) hydrochloride (1 M stock, H8602, Sigma-Aldrich), serotonin hydrochloride (50 mM stock, 14332, CAY), and L-adrenaline (epinephrine) (5 mM stock, A0173, TCI) were dissolved in 10 mM HCl. L-noradrenaline bitartrate monohydrate (1 M stock, A0906, TCI), sodium L-glutamate monohydrate (10 mM stock, G0188, TCI), 4-aminobutyric acid (100 mM stock, A0282, TCI), histamine (100 mM stock, 18111–71, Nacalai Tesque), acetylcholine chloride (10 mM stock, A6625, Sigma-Aldrich), R( +)-SCH 23390 hydrochloride (10 mM stock, D054, Sigma-Aldrich), and octopamine hydrochloride (10 mM stock, O0413, TCI) were each dissolved separately in distilled water. SKF 81297 hydrobromide (10 mM stock, 1447, TOCRIS), haloperidol hydrochloride (20 mM stock, 0931, TOCRIS), yohimbine hydrochloride (20 mM stock, 1127, TOCRIS), and tyramine (10 mM stock, A0302, TCI) were dissolved in DMSO. Compound solutions were then subdivided into aliquots and stored at − 20 °C until use. A working solution of 1 M DA was stored at 4 °C for 3 weeks prior to use.

Nakamoto C., Goto Y., Tomizawa Y., Fukata Y., Fukata M., Harpsøe K., Gloriam D.E., Aoki K, & Takeuchi T. (2021). A novel red fluorescence dopamine biosensor selectively detects dopamine in the presence of norepinephrine in vitro. Molecular Brain, 14, 173.

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Radioligand Binding Assay Protocol

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Dopamine hydrochloride and L-(-)-norepinephrine (+)-bitartrate salt monohydrate were purchased from Sigma. (-) quinpirole hydrochloride, clonidine hydrochloride, 7-OH-PIPAT maleate, RO-105824 dihydrochloride, RX821002 and yohimbine hydrochloride were purchased from Tocris. [³H]RX821002 (63.9 Ci/mmol), [³H]SCH 23390 (81.9 Ci/mmol) and [³H]YM-09151-2 (84.4 Ci/mmol) were from Perkin-Elmer. Pertussis toxin was purchased from Sigma.

Sánchez-Soto M., Casadó-Anguera V., Yano H., Bender B.J., Cai N.S., Moreno E., Canela E.I., Cortés A., Meiler J., Casadó V, & Ferré S. (2018). α2A- and α2C-Adrenoceptors as Potential Targets for Dopamine and Dopamine Receptor Ligands. Molecular neurobiology, 55(11), 8438-8454.

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Preparation of Psychoactive Drug Solutions

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Yohimbine hydrochloride (Tocris, UK) was dissolved in distilled water. Citalopram hydrobromide (Tocris, UK), buspirone hydrochloride (Sigma, UK) amphetamine (Sigma, UK) and alpha-flupenthixol (Sigma, UK) were dissolved in 0.9 % saline. FG7142 (β-carboline-3-carboxylic acid N-methylamide) and diazepam (both Sigma, UK) were dissolved in 10 % dimethyl sulphoxide (DMSO; BDH Laboratory Supplies, UK), 20 % cremophor EL (Sigma, UK) and 70 % of 0.9 % saline. All drugs were administered at a final volume of 1 ml/kg.

Phelps C.E., Mitchell E.N., Nutt D.J., Marston H.M, & Robinson E.S. (2015). Psychopharmacological characterisation of the successive negative contrast effect in rats. Psychopharmacology, 232(15), 2697-2709.

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Analgesic and Anti-inflammatory Protocol

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Atropine, carrageenan, acetylsalicylic acid, indomethacin, formaldehyde and L-nitro arginine methyl ester (L-NAME) were purchased from Sigma (St Louis, MO, USA). Yohimbine hydrochloride was purchased from Tocris (Ellisville, MO, USA). Tramadol, naloxone, amitriptyline hydrochloride and morphine sulfate were donated by Cristália Produtos Químicos e Farmacêuticos Ltda (Itapira, SP, Brazil). ASE was dissolved in distilled water (10 mg.ml⁻¹, stock solution).

Sudo R.T., Neto M.L., Monteiro C.E., Amaral R.V., Resende Â.C., Souza P.J., Zapata-Sudo G, & Moura R.S. (2015). Antinociceptive effects of hydroalcoholic extract from Euterpe oleracea Mart. (Açaí) in a rodent model of acute and neuropathic pain. BMC Complementary and Alternative Medicine, 15, 208.

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Pharmacological Manipulation of Neurochemicals

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Chen C., Jiang Z., Fu X., Yu D., Huang H, & Tasker J.G. (2019). Astrocytes Amplify Neuronal Dendritic Volume Transmission Stimulated by Norepinephrine. Cell reports, 29(13), 4349-4361.e4.

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Intravenous and Intraperitoneal Methamphetamine Administration

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Methamphetamine hydrochloride (purity 99%; METH, ‘ice’) was sourced from the Australian Government Analytical Laboratories (Pymble, NSW). For IVSA, METH was dissolved in saline (0.9%) at a dose of 0.1 mg/kg per 0.05 mL infusion and filtered through a Millipore syringe filter (0.45 µm). For intraperitoneal (IP) injection, METH was administered at a dose of 1 mg/kg and at a volume of 1 mL/kg. Yohimbine hydrochloride was sourced from Tocris Bioscience (Bristol, UK), was dissolved in distilled water at a dose of 0.625 mg/kg or 1.25 mg/kg and was administered at a volume of 2 mL/kg.

Webb P.I., Hill T.J., Everett N.A., Thornton J.L., Cornish J.L, & Baracz S.J. (2022). The effect of adolescent social isolation on vulnerability for methamphetamine addiction behaviours in female rats. Psychopharmacology, 239(4), 1129-1141.

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Pharmacological Agents for Neuroscience Research

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ARC-239, BRL-44408, clonidine hydrochloride, dexmedetomidine hydrochloride, efaroxan hydrochloride, guanabenz acetate, guanfacine hydrochloride, idazoxan hydrochloride, JP-1302, prazosin hydrochloride, rauwolscine hydrochloride, RS-79948, RX-821002, terazosin, UK-14304, WB-4101 and yohimbine hydrochloride were acquired from Tocris (Ellisville, MO). Atipamezole was from Orion Corporation (Espoo, Finland) and isoflurane was from Abbott Diagnostics (Chicago, IL). All other chemicals were obtained from Sigma-Aldrich (St. Louis, MO).

Biggane J.P., Xu K., Goldenstein B.L., Davis K.L., Luger E.J., Davis B.A., Jurgens C.W., Perez D.M., Porter J.E, & Doze V.A. (2022). Pharmacological characterization of the α2A-adrenergic receptor inhibiting rat hippocampal CA3 epileptiform activity: Comparison of ligand efficacy and potency. Journal of receptor and signal transduction research, 42(6), 580-587.

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Stimulants and Neuropeptide Effects

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Methamphetamine hydrochloride (METH) was purchased from the Australian Government Analytical Laboratories (Australia), oxytocin from China Peptides (China) and yohimbine hydrochloride from Tocris Bioscience (United Kingdom). For intraperitoneal (i.p.) injections, METH was injected at a volume of 1 ml/kg, whilst oxytocin and yohimbine were injected at 2 ml/kg.

Baracz S.J., Robinson K.J., Wright A.L., Turner A.J., McGregor I.S., Cornish J.L, & Everett N.A. (2022). Oxytocin as an adolescent treatment for methamphetamine addiction after early life stress in male and female rats. Neuropsychopharmacology, 47(8), 1561-1573.

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Evaluation of Oxaliplatin and Mitragynine

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Oxaliplatin was obtained from Temple University Hospital Pharmacy (Philadelphia, PA) as a 6 mg/ml concentration stock solution and diluted in sterile water (B. Braun Medical Inc., Irvine, CA). Mitragynine (MG) was obtained from Cayman Chemical Company (Ann Arbor, MI) with molecular weight and purity (>97.3 %) additionally confirmed by Dr. Allen Reitz (Fox Chase Chemical Diversity Center, Doylestown, PA). MG was dissolved in a vehicle of 20 % Tween 80 / 80 % sterile water. Naltrexone hydrochloride (NTX), prazosin hydrochloride (PRZ), and propranolol hydrochloride (PROP) were obtained from Sigma-Aldrich (St. Louis, MO) and dissolved in sterile water. Yohimbine hydrochloride (YOH) was purchased from Tocris Bioscience (Bristol, UK) and dissolved in sterile water. All drugs were administered intraperitoneally (IP) at a volume of 10 ml/kg.

Farkas D.J., Foss J.D., Ward S.J, & Rawls S.M. (2022). Kratom alkaloid mitragynine: Inhibition of chemotherapy-induced peripheral neuropathy in mice is dependent on sex and active adrenergic and opioid receptors. IBRO Neuroscience Reports, 13, 198-206.

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