The morphology of the nanoparticles was examined by SEM (Hitachi SU8030) (Chiyoda City, Tokyo, Japan) at an operating voltage of 1 kV. One drop of freshly prepared particle suspension was deposited onto the sample stub and left to dry in air and the dried sample was coated with chromium. For transmission electron microscopy analysis, a 30 kV high-resolution scanning transmission electron microscope (STEM)(Hitachi SU8030, Chiyoda City, Tokyo, Japan ) was used to examine the morphological characteristics of the OVA-loaded nanoparticles. One drop of sample suspension was placed on a 300 mesh copper grid and allowed to sit on the grid for 10 min until it air dried. Excess liquid was removed by using filter paper before observation on the STEM machine.
Su8030
Manufactured by Hitachi
Sourced in Japan, Germany, United States, United Kingdom
The SU8030 is a high-performance scanning electron microscope (SEM) manufactured by Hitachi. It is designed to provide high-resolution imaging and analysis capabilities for a wide range of applications. The SU8030 features a field emission gun (FEG) electron source, which enables high-resolution imaging with low accelerating voltages. It also offers advanced analytical capabilities, such as energy-dispersive X-ray spectroscopy (EDS) and electron backscatter diffraction (EBSD), for materials characterization.
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Lab products found in correlation
Conductive carbon adhesive tape, by Agar Scientific (4 mentions)
Double sided carbon adhesive tape, by Agar Scientific (3 mentions)
Nicolet is50, by Thermo Fisher Scientific (3 mentions)
Nano z, by Malvern Panalytical (2 mentions)
Noran system 7, by Thermo Fisher Scientific (2 mentions)
Axs d8, by Bruker (2 mentions)
Multimode 8 hr, by Bruker (2 mentions)
Ultima 4, by Rigaku (2 mentions)
Escalab 250xi, by Thermo Fisher Scientific (2 mentions)
K alpha, by Thermo Fisher Scientific (2 mentions)
S 4800 2, by Hitachi (2 mentions)
Jfc 1600, by JEOL (1 mentions)
Xplora, by Horiba (1 mentions)
Icap q instrument, by Thermo Fisher Scientific (1 mentions)
Icap 7600, by Thermo Fisher Scientific (1 mentions)
Tristar 2 3020, by Micromeritics (1 mentions)
Uv 2600, by Shimadzu (1 mentions)
Polyvinylpyrrolidone, by Thermo Fisher Scientific (1 mentions)
Sta 449 c jupiter, by Netzsch (1 mentions)
449 f3 jupiter, by Netzsch (1 mentions)
83 protocols using su8030
1
Morphology Analysis of OVA-loaded Nanoparticles
2
Scanning Electron Microscopy of Microneedle Arrays
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The MN arrays were mounted onto aluminium stubs using a double-sided carbon adhesive tape (Agar scientific, UK). Each MN array was examined by SEM (Hitachi SU 8030, Japan) using a low accelerating voltage (1.0 kV). A low accelerating voltage was used to avoid electrical charges on the MNs. The images of the MNs were captured digitally from a fixed working distance (11.6 mm) using different magnifications. Processing the images and measurements were conducted through the software ImageJ (Fiji package) (NIH image, Bethesda, MD, USA).
3
Structural Analysis of 3D-Printed BRS
4
Surface Morphology Analysis of Cellulose and Nanocellulose
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The surface morphology analysis of cellulose and nanocellulose products was studied with a field emission scanning electron microscope (FESEM; Hitachi SU8030, (Hitachi HTA, Schaumburg, IL, USA)) at an accelerating voltage of 5 kV. The samples were mounted on double-sided adhesive carbon tapes that stick on aluminum stubs, and coated with platinum using an auto-fine coater (JFC-1600, (JEOL, Ltd., Tokyo, Japan)) to improve conductivity and avoid over-charging. The elemental analysis of each sample was determined using EDX coupled with FESEM unit. The size and dimensions of yielded nanocellulose were further investigated by a transmission electron microscope (TEM; Tecnai G2 F20 Series, (FEI company, Hillsboro, OR, USA)) performed at a 200 kV acceleration voltage. To perform the TEM analysis, a nanocellulose suspension was treated with ultrasound for 3 min to separate agglomerated fibers. A droplet of the diluted suspension was deposited on a copper grid coated with a thin carbon film, and dried in a vacuum desiccator prior to analysis to ensure the samples were completely dry. The morphology and diameter of the yielded nanocellulose fibers were determined by analyzing the micrographs with ImageJ software (National Institutes of Health, New York, NY, USA).
5
Structural Characterization and SERS Analysis
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Structural characterization of diatom valves as well as of the ultrathin gold layer was performed with a Hitachi SU8030 scanning electron microscope, supported with a secondary electron detector.
For SERS measurements, about 7 µL of 1 mM Rhodamine 6G (R6G) in ethanol was dropped on top of the gold and left to dry at ambient conditions. SERS measurements and mapping were carried out with a backscattering configuration on a Horiba XploRA confocal Raman instrument equipped with a charge-coupled device (CCD) detector. The spectra acquisition was carried out using an excitation laser wavelength of 638 nm of ca. 40 mW power, in a spectral range of 500–2100 cm−1, with an integration time of 15 s per spectrum and averaged over 5 accumulations. Raman mapping was performed with a 1 μm step in the case of Aula and a 0.5 μm step in the cases of Cosc and Gomp, with an integration time of 1 s per step. For focusing the light, the 100x objective (NA = 0.9) was used, giving a beam size of approximately 0.5 μm. Grating was set to 1200 grooves/mm.
For SERS measurements, about 7 µL of 1 mM Rhodamine 6G (R6G) in ethanol was dropped on top of the gold and left to dry at ambient conditions. SERS measurements and mapping were carried out with a backscattering configuration on a Horiba XploRA confocal Raman instrument equipped with a charge-coupled device (CCD) detector. The spectra acquisition was carried out using an excitation laser wavelength of 638 nm of ca. 40 mW power, in a spectral range of 500–2100 cm−1, with an integration time of 15 s per spectrum and averaged over 5 accumulations. Raman mapping was performed with a 1 μm step in the case of Aula and a 0.5 μm step in the cases of Cosc and Gomp, with an integration time of 1 s per step. For focusing the light, the 100x objective (NA = 0.9) was used, giving a beam size of approximately 0.5 μm. Grating was set to 1200 grooves/mm.
6
Characterization of NU-1000 Metal-Organic Framework
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Crystallinity of each NU-1000 materials was verified with powder X-ray diffraction (PXRD) on a STOE STADI P with CuKα1 radiation and compared to a simulated NU-1000 pattern. Porosity of NU-1000 and cyt c@NU-1000 were measured using isothermal N2 adsorption at 77 K on Micromeritics Tristar II 3020. Pore size distributions (PSDs) were calculated by N2 DFT model with slit geometry. Prior to N2 adsorption measurements, cyt c@NU-1000 was activated with supercritical CO2 and at 80°C under vacuum overnight on Micromeritics Smart VacPrep instrument. UV-vis measurements were performed on Shimadzu UV-2600 in a quartz cuvette. SEM images and SEM-EDS line scans were taken on Hitachi SU8030 equipped with Oxford AZtec software after 9 nm of Os plasma coating. ICP-OES was performed on Thermo iCap7600, and ICP-MS was performed on Thermo iCapQ instrument. DLS and zeta potential were measured by Zetasizer (Malvern Instruments Ltd, NanoZS).
7
Thin Film Thickness Characterization
8
Electrospray Deposition of Carbon Nanotubes
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Commercial multi-walled carbon nanotubes (MWCNTs, 15 ± 5 nm diameter, 1–5 μm length) were purchased from Nanolab, Inc. These CNTs were not subject to any explicit chemical treatment for functionalization. In-house CsH2PO4 was synthesized by dissolving stoichiometric quantities of Cs2CO3 and H3PO4 (85% assay) in deionized water, followed by a methanol-induced precipitation. The resulting precipitate was dried at 120 °C for 12 h. Untreated Toray carbon paper (TGP-H-120, Fuel Cell Earth, LLC.) was used as the current collector in electrochemical cells and as the substrate for electrospray deposition. Polyvinylpyrrolidone (Alfa Aesar, Mw ∼ 8000 g mol–1) and Nanosperse AQ (Nanolab, Inc.) were used as dispersants for suspending carbon nanotubes in aqueous solutions in the electrospray step. Scanning electron microscopy images were collected at the Department of Geological and Planetary Sciences at Caltech (ZEISS 1550VP FESEM) and at Northwestern University's Atomic and Nanoscale Characterization Experimental Center (Hitachi SU8030). Thermogravimetric analysis was conducted on Netzsch STA 449 C Jupiter and Netzsch STA 449 F3 Jupiter thermal analyzers. X-Ray powder diffraction was performed using a PANalytical X'Pert PW3040-PRO (Cu Kα). Raman spectra were collected with a Renishaw M1000 Micro Raman Spectrometer System, using a green laser at 514.5 nm.
9
Microstructural Analysis of 3D-Printed Tablets
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SEM (Hitachi SU8030, Tokyo, Japan) was utilised to investigate the internal microstructures of the 3D-printed tablets, as well as the laser intensity effect on the tablet porosity and permeability. The tablets were kept secured on an aluminium stub with a conductive carbon adhesive tape (Agar Scientific, Stansted, UK). The tablets were then examined via SEM, and images were captured by an electron beam accelerating voltage of 1 KV and a magnification of 30×.
10
Characterizing PEDOT-POCO Film Composition
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Samples were dried overnight at room temperature and then coated in carbon with a Denton III Desk Sputter Coater. EDS was performed on a Hitachi SU8030 to visualize the distribution of sulfur throughout the cross-section of the PEDOT-POCO film. Data was collected and processed using Aztec software.
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