Agilent 1100 series uv lamp

ETO concentrations in samples were measured by HPLC-UV (Hewlett Packard series 1100 HPLC Pump combined with Agilent Technologies 1200 Series Autosampler and a ChemStation software (Agilent Technologies, B.04.03 version, Santa Clara, CA, USA). Injection volume was 50 µL and UV detection wavelength was 254 nm (Agilent 1100 Series UV lamp). Linearity was demonstrated between 50 µM and 500 µM with calibrated curves prepared in mobile phase from a ETO methanol stock solution (7 mM). Limit of detection (LOD) of and limit of quantification (LOQ) were 2.2 µM and 6.6 µM, respectively. The mobile phase was a mixture of 20:80 (v/v) water:acetonitrile both containing 0.1% (v/v) trifluoroacetic acid. After 2.3 min, the peak signal of ETO was observed using a reversed-phase C-18 column (Bondapak 250 mm × 4.6 mm—5 micron) and a 1 mL/min flow rate.

All samples derived from the GIS studies were analyzed for loratadine by HPLC-UV (Hewlett Packard series 1100 HPLC Pump combined with Agilent Technologies 1200 Series Autosampler (Santa Clara, CA, USA). A volume of 100 µL was injected into the HPLC system connected to a UV-lamp that was able to detect loratadine at a wavelength of 248 nm (Agilent 1100 Series UV lamp). An isocratic run with a mixture of 70% acetonitrile and 30% purified water was used to detect loratadine using a C-18 column (Kinetex C18 HPLC column, 250 × 4.60 mm^‒5 micron, Phenomenex, Golden, CO, USA) and a 1 mL/min flow rate. Calibration curves were made in methanol, based on a stock solution of loratadine in methanol (0.1 mg/mL). Linearity was observed between 40 µg/mL and 0.156 µg/mL with a regression coefficient of at least 0.995 between the AUC of the obtained peaks versus the spiked concentrations. The peaks were integrated using ChemStation software (Agilent Technologies, B.04.03 version, Santa Clara, CA, USA).

Solubility samples were analyzed by HPLC–UV (Hewlett Packard series 1100 HPLC Pump, Santa Clara, CA, USA), combined with Agilent Technologies 1200 Series Autosampler (Santa Clara, CA, USA). A volume of 5 µL was injected into the HPLC system connected to a UV lamp that was able to detect ibuprofen at a wavelength of 220 nm (Agilent 1100 Series UV lamp, Santa Clara, CA, USA). An isocratic run containing 70% acetonitrile (VWR International, West Chester, PA, USA) and 30% purified water (both containing 0.1% TFA) was used to detect ibuprofen at a retention time of 2.9 min using a reversed-phase C-18 column (Eclipse Plus C18, 4.6 × 150 mm, 5.5 µm, Agilent Technologies) and a 1 mL/min flow rate. The calibration curve was made in methanol based on a stock solution of ibuprofen in methanol (1 mM). Linearity was observed between 10.32 µg/mL and 0.32 µg/mL. The observed peaks were integrated using ChemStation software (Agilent Technologies, B.04.03 version). The developed analytical method met the FDA requirements for bioanalytical method validation [21 ].