MTT solution, mouse monoclonal anti-β-actin, NA, NMN and NAD were obtained from Sigma (St. Louis, MO, USA). The antibodies against (PAK4, p-PAK4, β-catenin, p-β-catenin, cycline D1, c-Myc, β-actin (rabbit), PARP, Sirtuin 1) were from cell signaling Technology, Inc. (Beverly, MA, USA). Goat anti-mouse and goat anti-rabbit HRP conjugated IgG were obtained from Bio-Rad (Hercules, CA). Anti-NAMPT was from Bethyl Laboratories (Montgomery, TX, USA), anti-NAPRT1 was from Proteintech (Rosemont, IL 60018, USA). ECL Plus solution was from Thermo-Fisher Scientific (Waltham MA, USA). KPT-9274 and its vehicle were from Karyopharm Therapeutics (Newton, MA, USA). FK866 was from TOCRIS Biosciences. Sunitinib was obtained from LC laboratories (Woburn, MA).
Fk866
Manufactured by Bio-Techne
Sourced in United Kingdom
FK866 is a small-molecule inhibitor that selectively targets the enzyme nicotinamide phosphoribosyltransferase (NAMPT). NAMPT is a key enzyme involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+), an essential cofactor in various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Goat anti mouse and goat anti rabbit hrp conjugated igg, by Bio-Rad (1 mentions)
Mtt solution, by Merck Group (1 mentions)
Anti nampt, by Fortis Life Sciences (1 mentions)
C myc, by Cell Signaling Technology (1 mentions)
Mouse monoclonal anti β actin, by Merck Group (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
β catenin, by Cell Signaling Technology (1 mentions)
P pak4, by Cell Signaling Technology (1 mentions)
Sirtuin 1, by Cell Signaling Technology (1 mentions)
Ecl plus solution, by Thermo Fisher Scientific (1 mentions)
P β catenin, by Cell Signaling Technology (1 mentions)
Sunitinib, by LC Laboratories (1 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Cisplatin, by Bio-Techne (1 mentions)
2 protocols using fk866
1
Protein Expression Quantification Protocol
2
Evaluating NAMPT Inhibitors in Cholangiocarcinoma
Check if the same lab product or an alternative is used in the 5 most similar protocols
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HuCCT1, EGI, and KMCH CCA cells were grown in DMEM (Thermo Fisher Scientific, Waltham, MA, USA) containing 10% FBS and 1% penicillin/streptomycin. Additionally, normal human cholangiocyte cell lines (H69 and NHC) were employed in this work. 0.2–50 nm of FK866 (Tocris Bioscience, Bristol, UK) and 0.2–20 μM of Cisplatin (Tocris Bioscience, Bristol, UK) were applied to the cells at different concentrations for 2–3 days. Lipofectamine RNAiMAX (Thermo Fisher Scientific) was used to transfect NAMPT-siRNA (10 nM) (Dharmacon Inc., Lafayette, CO, USA) and non-Target-siRNA (NT) in the HuCCT1 cells.
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