Second antibody conjugated hrp goat anti mouse and goat anti rabbit

d-Gal and GlcN were procured from Sigma-Aldrich (Darmstadt, Germany). 3-Methyladenine (3-MA) was purchased from Selleckchem (Houston, TX, USA). ELISA kits for cytokines IL-1β and IL-6 were procured from Boster (Wuhan, Hubei, China). Malonaldehyde (MDA) and superoxide dismutase (SOD) assay kit was purchased from Jiancheng Bioengineering (Nanjing, Jiangsu, China).The cDNA was synthesized by reverse transcription using a cDNA synthesis kit (Takara, Japan), and was analyzed with SYBR Green Real-time PCR kit (Novizan Biotechnology, Nanjing, Jiangsu, China).The antibodies included LC3, Beclin-1, P62, RUNX2, OCN, BMP2, Bcl-2, BAX and Cleaved Caspase-3 were purchased from Cell Signaling Technology (Danvers, MA, USA), and the antibodies to Ki-67 and p16 were all obtained from Beyotime (Shanghai, China). The antibody to β-actin and the second antibody conjugated HRP (goat-anti-mouse and goat-anti-rabbit) were purchased from Proteintech (Chicago, IL, USA). The Alkaline phosphatase (ALP) staining kit, Goldner trichrome staining kit and Tartrate-resistant acid phosphatase (TRAP) staining kit were purchased from Solarbio (Beijing, China).