Isolated EVs were concentrated by ultrafiltration on 100-K filters (Millipore), adhered to carbon-coated 200 mesh Cu grids, and labeled with CD63 antibody (BD Biosciences). Grids were incubated with secondary 10 nm gold-conjugated antibody with matching isotypes. Following a wash in 0.1 M phosphate buffer, pH 7.0, grids were fixed with 4% paraformaldehyde + 1% glutaraldehyde and negative stained with 1% phosphotungstic acid. Micrographs were acquired using a JEOL1400 Plus transmission electron microscope operating at 80 kV (Peabody, MA, USA).
100 k filters
Manufactured by Merck Group
The 100-K filters are high-performance filtration devices designed for efficient separation of particles, microorganisms, and other contaminants from various liquid or gas samples. The core function of these filters is to provide reliable and consistent filtration, ensuring the purity and quality of the processed media.
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Lab products found in correlation
2 protocols using 100 k filters
1
Visualizing Extracellular Vesicles by TEM
2
Immunogold Labeling of Extracellular Vesicles
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Isolated L-EVs were concentrated by ultrafiltration on 100K filters (Millipore), adhered to carbon-coated 200 mesh Cu grids and immunogold labeled with Annexin A2 (Santa Cruz, sc-28385) or S100A9 antibody (Sigma, HPA004193). Following primary antibody stain, grids were incubated with secondary 10nm gold conjugated antibody or secondary antibody alone. Following a wash in 0.1 M phosphate buffer, pH 7.0, grids were fixed with 4% paraformaldehyde + 1% glutaraldehyde and negatively stained with 1% phosphotungstic acid. Micrographs were acquired using a JEOL1400 Plus transmission electron microscope operating at 80 kV (Peabody, MA).
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