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Echomri 3 in 1 body composition analyzer

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The EchoMRI 3-in-1 Body Composition Analyzer is a lab equipment product that provides a comprehensive analysis of body composition. It measures total body fat, lean body mass, and total body water through a non-invasive scanning process.

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Lab products found in correlation

Comprehensive lab animal monitoring system clams, by Columbus Instruments (3 mentions) Humulin r, by Eli Lilly (2 mentions) Alphatrak 2 blood glucose monitoring system, by Abbott (2 mentions) D12451, by Research Diets (1 mentions) Comprehensive lab animal monitoring system, by Columbus Instruments (1 mentions) Mouse insulin elisa kit, by Crystal Chem (1 mentions)

Close spelling variants of this product

EchoMRI 3-in-1 composition analyzer EchoMRI body composition analyzer EchoMRI 3-in-1 analyzer EchoMRI 3-in-1 Body Composition Analyzer EchoMRI 3-1 The 3-in-1 EchoMRI

7 protocols using echomri 3 in 1 body composition analyzer

1

Comprehensive Metabolic Profiling in Aging Mice

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To assess multiple metabolic parameters in aging and young mice, CLAMS (Columbus Instruments) metabolic cages was employed as described by Kang and colleagues45 (link). Briefly, mice were transferred to individual metabolic cages without bedding, with free access to standard chow diet and tap water and acclimated for 72 h before switching to HFD and starting 7 days of daily rGDF11, rGDF8 or saline injection. Mice were subjected to non-invasive daily monitoring of gas exchange, physical activity, and manual food weight measurements performed by a balance connected to each CLAMS metabolic cage as a readout for food intake and an Echo-MRI 3-in-1 Body composition analyzer (Echo Medical Systems) was employed to measure total body fat and lean mass. Respiratory exchange ratios (RER) were calculated as the ratio of carbon dioxide produced to oxygen consumed and energy expenditure (kcal/hour) were calculated from gas exchange. Physical activity was determined according to beam breaks within a grid of photosensors built into the cages. Total activity was defined as the total number of beam breaks, whereas ambulatory activity was determined as successive beam breaks within the grid45 (link).
Walker R.G., Barrandon O., Poggioli T., Dagdeviren S., Carroll S.H., Mills M.J., Mendello K.R., Gomez Y., Loffredo F.S., Pancoast J.R., Macias-Trevino C., Marts C., LeClair K.B., Noh H.L., Kim T., Banks A.S., Kim J.K., Cohen D.E., Wagers A.J., Melton D.A, & Lee R.T. (2020). Exogenous GDF11, but not GDF8, reduces body weight and improves glucose homeostasis in mice. Scientific Reports, 10, 4561.
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2

Dietary Effects on Metabolic Outcomes in Mice

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Experimental feeding of offspring began at weaning on PND 21. Mice were housed by sex, 3–6 per cage depending on litter size, and given ad libitum access to food and water. Offspring diet was either low-fat diet (LFD – 10% kCal fat; Research Diets D12450H) or a standard high-fat diet (HFD – 45% kCal fat; Research Diets D12451). Body weight and food intake (per cage food intake) were recorded weekly for 25 weeks followed by body composition measurements using an EchoMRI 3-in-1 Body Composition Analyzer (Echo Medical Systems, Houston, TX, USA) and calorimetric and activity measurements (48 h run) via Columbus Instruments’ Comprehensive Lab Animal Monitoring System (Columbus Instruments, Inc., Columbus, OH, USA). A glucose tolerance test (GTT), following a 5 h fast, was administered via intraperitoneal (ip) injection of 2 g/kg glucose in 0.9% saline solution. Blood glucose (BG) from tail blood was measured with an AlphaTrak 2 Blood Glucose Monitoring System (Abbott Laboratories, Abbott Park, IL, USA) pre-injection and 15, 30, 60, 90, 120, and 180-min post-injection. An insulin tolerance test (ITT) following a 4 h fast involved an injection of 0.75 U/kg insulin (Humulin R, Lilly, Indianapolis, IN, USA) in 0.9% saline solution and followed the same BG measurement scheme as GTT. Mice were given 4 days of rest each between CLAMS, GTT, and ITT.
Mamounis K.J., Shvedov N.R., Margolies N., Yasrebi A, & Roepke T.A. (2019). The effects of dietary fatty acids in the physiological outcomes of maternal high-fat diet on offspring energy homeostasis in mice. Journal of developmental origins of health and disease, 11(3), 273-284.
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3

Metabolic Phenotyping of Mice

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Experimental feeding began at 12 weeks of age. See Table 2 for timeline. Mice were housed 3 per cage and given ad libitum access to food and water. Body weight and food intake (per cage food intake) were recorded weekly for 12 weeks followed by body composition measurements using an EchoMRI 3-in-1 Body Composition Analyzer (Echo Medical Systems, Houston, TX, USA) and calorimetric and activity measurements (48 h run) via Columbus Instruments’ Comprehensive Lab Animal Monitoring System (CLAMS) (Columbus Instruments, Inc., Columbus, OH, USA). A glucose tolerance test (GTT), following an overnight fast, was administered via intraperitoneal (ip) injection of 2g/kg glucose in 0.9% saline solution. Blood glucose (BG) from tail blood was measured with an AlphaTrak 2 Blood Glucose Monitoring System (Abbott Laboratories, Abbott Park, IL, USA) pre-injection and 15, 30, 60, 90, 120 and 180 minutes post-injection. An insulin tolerance test (ITT) following a 5 h fast involved an injection of 0.5 U/kg insulin (Humulin R, Lilly, Indianapolis, IN, USA) in 0.9% saline solution and followed the same BG measurement scheme as GTT. Mice were given 4 days of rest each between CLAMS, GTT, and ITT.
Mamounis K.J., Yasrebi A, & Roepke T.A. (2016). Linoleic acid causes greater weight gain than saturated fat without hypothalamic inflammation in the male mouse. The Journal of nutritional biochemistry, 40, 122-131.
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4

Comprehensive Metabolic Phenotyping in Mice

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To assess multiple metabolic parameters, CLAMS (Columbus Instruments) metabolic cages was employed as previously described [23 (link)]. Briefly, mice were transferred to individual metabolic cages without bedding, with free access to food and tap water and acclimated for 48 h before starting measurements. Mice were subjected to non-invasive monitoring of gas exchange, physical activity and food intake and an Echo-MRI 3-in-1 Body composition analyzer (Echo Medical Systems) was employed to measure total body fat and lean mass. Respiratory exchange ratios (RER) were calculated as the ratio of carbon dioxide produced to oxygen consumed and energy expenditure (kcal/hour) were calculated from gas exchange. Values of energy expenditure were adjusted for lean body mass by ANCOVA. Physical activity was determined according to beam breaks within a grid of photosensors built into the cages. Total activity was defined as the total number of beam breaks. To assess food consumption and determine the cumulative amount of food eaten, a balance connected to each cage in the CLAMs apparatus was employed.
Carroll S.H., Zhang E., Wang B.F., LeClair K.B., Rahman A., Cohen D.E., Plutzky J., Patwari P, & Lee R.T. (2017). Adipocyte arrestin domain-containing 3 protein (Arrdc3) regulates uncoupling protein 1 (Ucp1) expression in white adipose independently of canonical changes in β-adrenergic receptor signaling. PLoS ONE, 12(3), e0173823.
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5

Longitudinal Metabolic Phenotyping in Knockout Mice

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From 5 to 25 weeks of age, females were weighed weekly without monitoring the estrous cycle as KO and KIKO do not exhibit a normal estrous cycle34 (link),35 (link). At the end of 25 weeks, body composition was measured using an EchoMRI 3-in-1 Body Composition Analyzer (Echo Medical Systems, Houston, TX, USA) followed by a 48h run in a Comprehensive Lab Animal Monitoring System (CLAMS; Columbus Instruments, Columbus, OH, USA) to measure metabolic parameters and activity (X- and Z-plane). After CLAMS, a glucose tolerance test (GTT) was performed on each female following standard protocols28 (link). Immediately after baseline, females were injected intraperitoneally (i.p.) with glucose (2.0 g/kg body weight) and individually housed in clean cages. After sufficient recovery (~3-4 d), an insulin tolerance test (ITT) was performed after a 5h fast with an i.p. injection of insulin (0.75 units/kg). Blood samples were collected from the tail in individual cages at 15, 30, 60, 90, and 120 min post-injection for both the GTT and ITT. See Supplemental Figure S1 for a graphical illustration of the maternal and adult experimental design.
Roepke T.A., Yasrebi A., Villalobos A., Krumm E.A., Yang J.A, & Mamounis K.J. (2019). The loss of ERE-dependent ERα signaling potentiates the effects of maternal high-fat diet on energy homeostasis in female offspring fed an obesogenic diet. Journal of developmental origins of health and disease, 11(3), 285-296.
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6

Comprehensive Metabolic Profiling in Mice

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Body composition was assessed using the EchoMRI 3-in-1 body composition analyzer (Echo Medical Systems, Houston, TX). The Comprehensive Lab Animal Monitoring System (CLAMS; Columbus Instruments, Columbus, OH), an indirect calorimeter, was used to measure O2 consumption (v.O2), CO2 production (v.CO2), and RER (v.CO2/v.O2) at 25°C. Energy expenditure was estimated according to CLAMS energy equation; EE (Cal/h) = [3.815 × v. CO2 + 1.232 × V.O2]. Ambulatory activity was also measured by an activity monitor using IR photocells to calculate activity. Mice were maintained on their respective dosing protocols and housed in the system for 7 days. The last 24-h epoch (dosing Day 28) was used for the analysis [30 (link)].
Kshatriya D., Li X., Giunta G.M., Yuan B., Zhao D., Simon J.E., Wu Q, & Bello N.T. (2019). Phenolic-enriched raspberry fruit extract (Rubus idaeus) resulted in lower weight gain, increased ambulatory activity, and elevated hepatic lipoprotein lipase and heme oxygenase-1 expression in male mice fed a high-fat diet.. Nutrition research (New York, N.Y.), 68, 19-33.
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7

Tissue NO Content and Metabolic Assessment

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Tissue NO content was measured by using an electron spin resonance spectroscopy (ESR) technique, as previously described (21 (link),31 (link)). Insulin levels were determined using a mouse insulin ELISA kit (Crystal Chem, Downers Grove, IL). Determinations of body lean and fat mass were made in conscious mice using quantitative magnetic resonance (EchoMRI 3-in-1 body composition analyzer; Echo Medical Systems, Houston, TX) in the University of Washington Nutrition Obesity Research Center. Hepatic triglyceride (TG) content was enzymatically measured in liver lysates as previously described (32 (link)).
Lee W.J., Tateya S., Cheng A.M., Rizzo-DeLeon N., Wang N.F., Handa P., Wilson C.L., Clowes A.W., Sweet I.R., Bomsztyk K., Schwartz M.W, & Kim F. (2015). M2 Macrophage Polarization Mediates Anti-inflammatory Effects of Endothelial Nitric Oxide Signaling. Diabetes, 64(8), 2836-2846.
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