A total of 4 eggs from each treatment (1 egg/replicate) were collected and broken to obtain the eggshell samples before the end of the experimental period. Cleaned eggshell samples were dried in an oven at 95 °C for 24 h, ground into a fine powder, and kept in a dry box until analysis. The dried right tibia was finely ground using an electric grinder. To observe the surface morphology of both the ground eggshell and the tibia specimens, scanning electron microscopy (SEM; model JSM-5410LV, JEOL Ltd., Japan) was performed using high vacuum mode at a working distance of 20 mm and an accelerating voltage of 20 kV. In addition, the quantitative analysis of calcium and phosphorus in the eggshell and tibia bone was conducted by energy-dispersive X-ray spectroscopy (EDS; model Link ISIS 300, Oxford Instruments Ltd., UK), combining SEM with the backscattered electron.
Link isis 300
Manufactured by Oxford Instruments
Sourced in United Kingdom
The Link ISIS 300 is a versatile and advanced laboratory equipment designed for a range of scientific applications. It provides reliable and precise measurements, serving as a core component in various research and analysis workflows. The detailed specifications and intended use of this product are not available within the scope of this request.
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Lab products found in correlation
Jsm 5410lv, by JEOL (1 mentions)
Zetasizer nano z, by Malvern Panalytical (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride edc, by Merck Group (1 mentions)
H 7650, by Hitachi (1 mentions)
Uv 2450, by Shimadzu (1 mentions)
S 3500, by Hitachi (1 mentions)
Phi 5000 versaprobe, by Physical Electronics (1 mentions)
Jem 2000fx electron microscope, by JEOL (1 mentions)
Jem 1010 transmission electron microscope, by JEOL (1 mentions)
4 protocols using link isis 300
1
Eggshell and Tibia Bone Analysis
2
Characterization of Fluorescent Nanobeads for IgE Detection
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Carboxyl-capped fluorescent nanobeads embedded with CdSe/ZnS QDs (QBC610) were obtained from Kundao Biotech (Shanghai, China). HDM natural D. pteronyssinus allergen 1 (nDer p 1) was purchased from Indoor Biotechnologies (Cardiff, UK). Goat anti-human IgE antibody, bovine serum albumin (BSA) and N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) were provided by Sigma-Aldrich (Darmstadt, Germany). The rabbit IgG, nitrocellulose membrane, sample pad, adhesion plastic back sheet and adsorption pad were obtained from Shanghai Kinbio Tech Co. Ltd (Shanghai, China).
Absorption (Ab) spectra were acquired with an ultraviolet visible (UV-Vis) spectrophotometer (UV-2450; Shimadzu, Kyoto, Japan), and fluorescent spectra were recorded on a fluorescence spectrometer (LS-55; PerkinElmer, Waltham, MA, USA). The morphology and size of QDs were analyzed with a transmission electron microscope (TEM; H-7650, Hitachi, Tokyo, Japan) at an accelerating voltage of 100 kV. The hydrodynamic diameters and size distribution of QDNBs were determined by a dynamic light scattering (DLS) system (Zetasizer Nano ZS; Malvern Instruments Ltd, Malvern, UK). The elemental analysis of QDNs were performed using scanning electron microscopy (S-3500, Hitachi) equipped with an energy-dispersive X-ray (EDX) spectrometer (Link ISIS 300; Oxford Instruments, Oxford, UK).
Absorption (Ab) spectra were acquired with an ultraviolet visible (UV-Vis) spectrophotometer (UV-2450; Shimadzu, Kyoto, Japan), and fluorescent spectra were recorded on a fluorescence spectrometer (LS-55; PerkinElmer, Waltham, MA, USA). The morphology and size of QDs were analyzed with a transmission electron microscope (TEM; H-7650, Hitachi, Tokyo, Japan) at an accelerating voltage of 100 kV. The hydrodynamic diameters and size distribution of QDNBs were determined by a dynamic light scattering (DLS) system (Zetasizer Nano ZS; Malvern Instruments Ltd, Malvern, UK). The elemental analysis of QDNs were performed using scanning electron microscopy (S-3500, Hitachi) equipped with an energy-dispersive X-ray (EDX) spectrometer (Link ISIS 300; Oxford Instruments, Oxford, UK).
3
Membrane Composition Analysis via EDS and XPS
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The chemical composition of the membrane is monitored by Energy Dispersive X-ray Spectroscopy, EDS (LINK ISIS 300, Oxford Instruments, UK) and X-ray photoelectron spectroscopy, XPS (PHI 5000 VersaProbe, Physical Electronics, Inc., Chanhassen, MN, USA) with monochromatized Mg Kα line with energy 1253.6 eV). Studies were carried out with the membranes conditioned in 10−1 mol L−1 NaHCO3 and non-conditioned membranes.
4
Transmission Electron Microscopy Protocol
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Sections of plant tissues of approximately 1 mm3 were fixed in the Electron Microscopy Service of the Centro de Biología Molecular Severo Ochoa (CBMSO) with the method described in Fuente et al. [6 (link)]. Samples were observed in the same service with a JEM-1010 transmission electron microscope and in the Centro Nacional de Microscopía Electrónica de Madrid with a JEM 2000FX electron microscope (JEOL, Tokyo, Japan) operated at 200 kV, coupled with an energy dispersive X-ray microanalysis instrument LINK ISIS 300 (Oxford Instruments, Oxford, UK).
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