A total of 2 mg dry products extracted from Massilia sp. UMI-21, E. coli DH5α-pETDuet1-phaA2phaB1-phaC1 and DH5α-pETDuet1-phaA2phaB1phaC1-vgb were mixed with potassium bromide (KBr) in 1:50 ratio to form samples and qualitative characterized by FT-IR analysis, which was carried out in IRAffinity-1 S instrument (SHIMADZU, Japan) for 15 scans and a resolution of 1 cm− 1.
Iraffinity 1s instrument
Manufactured by Shimadzu
Sourced in Japan
The IRAffinity-1S is an FTIR (Fourier Transform Infrared) spectrometer designed for qualitative and quantitative analysis of organic and inorganic compounds. It features a compact design, high-speed scanning, and a sealed and desiccated optical system to provide stable and reliable performance.
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5 protocols using iraffinity 1s instrument
1
FT-IR Analysis of Bioplastic Precursors
2
Characterization of Lignin Aggregates
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Lignin aggregates
in DMF and chloroform solutions were evaluated by a dynamic light
scattering (DLS) method using a ζ-potential and a particle size
analyzer (ELSZ2000ZS; Otsuka Electronics Co., Ltd., Osaka, Japan).
In addition, each solution was cast onto a glass coverslip and dried
in air for 1 week to remove the solvent prior to conducting scanning
electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy
analysis. SEM observations were performed using an SU-6600 instrument
(Hitachi High-Technologies Corporation, Tokyo, Japan). Samples were
placed on an SEM stub, coated in gold, and then analyzed at an accelerating
voltage of 5 kV. FT-IR spectra were recorded in the attenuated total
reflectance (ATR) mode (IRAffinity-1S instrument, Shimadzu Co., Kyoto,
Japan).
in DMF and chloroform solutions were evaluated by a dynamic light
scattering (DLS) method using a ζ-potential and a particle size
analyzer (ELSZ2000ZS; Otsuka Electronics Co., Ltd., Osaka, Japan).
In addition, each solution was cast onto a glass coverslip and dried
in air for 1 week to remove the solvent prior to conducting scanning
electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy
analysis. SEM observations were performed using an SU-6600 instrument
(Hitachi High-Technologies Corporation, Tokyo, Japan). Samples were
placed on an SEM stub, coated in gold, and then analyzed at an accelerating
voltage of 5 kV. FT-IR spectra were recorded in the attenuated total
reflectance (ATR) mode (IRAffinity-1S instrument, Shimadzu Co., Kyoto,
Japan).
3
Comprehensive Physicochemical Characterization
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Several techniques were employed to characterize the synthesized samples' physicochemical properties. X‐ray diffraction (XRD) patterns were obtained using an Empyrean Panalytical diffractometer with Cu Kα radiation at 45 kV/35 mA. The powders' local composition, morphology, and grain size were examined using scanning electron microscopy (SEM) (Supra 40 VP Column Gemini Zeiss) operating at 40 KeV, coupled with an EDS manifolder for quantitative elemental composition analysis. Fourier‐transform infrared (FTIR) spectroscopy was conducted using the SHIMADZU IRAffinity‐1S instrument to identify the functional groups present in the molecules. The samples were analyzed in the frequency range of 400–4000 cm−1 with a resolution of 4 cm−1. Light absorption properties of the samples were studied by UV–vis spectroscopy. The diffuse reflectance UV–vis spectra (UV–vis DRS) were recorded on a Varian spectrometer model Cary 100 equipped with an integrating sphere and using BaSO4 as a reference.
4
Lignin Characterization from Biomass
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To characterize the lignin structure of BM powder from SL and l -tartaric acid, lignin was isolated using a poor solvent, water. Regarding the notation used for the isolated lignin, the lignin isolated from BM-SLTA(10%) was denoted as IL-BM-SLTA(10%), as shown in Scheme 1 . The isolated lignin was analyzed using FT-IR spectroscopy and GPC. FT-IR spectra were recorded using the KBr pellet method (IRAffinity-1S instrument, Shimadzu Co., Kyoto, Japan). GPC was performed using an LC-10A instrument (Shimadzu, Kyoto, Japan) under the following conditions: Column, Shodex KF-801 + KF-802 + KF-802.5 + KF-803 (Showa Denko, Tokyo, Japan); eluent, tetrahydrofuran (THF); flow rate, 0.6 mL min−1; column temperature, 50 °C; detector, UV light at 280 nm. For comparison, polystyrene standards (molecular weights (MWs) of 162, 580, 1270, 2960, and 5000) were used. Prior to GPC analysis, lignin was acetylated to improve its solubility in THF in accordance with the general method.18
5
Synthesis and Characterization of Oxazoline Precursors
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General Experimental Details. Melting points were determined using a Gallenkamp 50W melting point apparatus and are uncorrected. IR spectra were measured using the ATR techique on a Shimadzu IRAffinity 1S instrument. NMR spectra were recorded on solutions in CDCl 3 at 400 MHz for 1 H and at 100 or 125 MHz for 13 C using Bruker AV400 or AVIII 500 spectrometers. HRMS measurements were made using a Micromass LCT spectrometer with electrospray ionisation. THF was dried over sodium wire.
Preparation of starting oxazolines. (S)-4-Benzyl-2methyloxazoline 5a and (S)-4-isopropyl-2-methyloxazoline 5b were prepared from ethyl acetimidate hydrochloride by respective reaction with (S)-phenylalaninol and (S)-valinol as previously reported, 5 while (S)-2,4-dibenzyloxazoline 5c was similarly prepared, 13 using ethyl 2-phenylacetimidate hydrochloride 14
Preparation of starting oxazolines. (S)-4-Benzyl-2methyloxazoline 5a and (S)-4-isopropyl-2-methyloxazoline 5b were prepared from ethyl acetimidate hydrochloride by respective reaction with (S)-phenylalaninol and (S)-valinol as previously reported, 5 while (S)-2,4-dibenzyloxazoline 5c was similarly prepared, 13 using ethyl 2-phenylacetimidate hydrochloride 14
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