Elisa

Manufactured by Diapharma

ELISA (Enzyme-Linked Immunosorbent Assay) is a versatile analytical technique used to detect and quantify specific substances, such as proteins, hormones, or antibodies, in a sample. It utilizes the principle of antigen-antibody interaction and an enzyme-based detection system to produce a measurable signal. The core function of ELISA is to provide a sensitive and reliable method for the identification and measurement of target analytes in complex biological samples.

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Lab products found in correlation

Glucose oxidase method, by Beckman Coulter (1 mentions)

3 protocols using elisa

Blood Glucose, Insulin, and PAI-1 Measurement

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Blood samples were collected in heparinized syringes. Plasma glucose was measured with the glucose oxidase method (Beckman Instruments, Fullerton, CA). Immunoreactive insulin and leptin was determined by RIA (Millipore, St. Louis, MO). Plasma PAI-1 activity was measured by ELISA (DiaPharma Group, Inc., West Chester, OH). All samples were run in duplicate with CV<10%.

McMillin S, & Ryan A. (2014). Plasminogen Activator Inhibitor-1, Body Fat and Insulin Action in Aging Women. Annals of gerontology and geriatric research, 1(2), 1006.

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Plasma Cytokeratin 18 Quantification

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Plasma cytokeratin 18 (CK-18) whole (M65®) and caspase-cleaved fragments (M30 Apoptosense®) were measured by ELISA (diaPharma, Columbus, OH). Clinical chemistry analysis was performed at the University of Louisville Hospital laboratory. Values were measured by clinical chemistry analyzer. Statistical analysis was performed by student’s t-test using GraphPad Prism. Statistical significance was set as p ≤ 0.05. Results are reported ad means ± standard deviation.

Guardiola J.J., Beier J.I., Falkner K.C., Wheeler B., McClain C.J, & Cave M. (2016). Occupational exposures at a polyvinyl chloride production facility are associated with significant changes to the plasma metabolome. Toxicology and applied pharmacology, 313, 47-56.

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Quantifying ADAMTS13 Activity and Inhibitors

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Plasma ADAMTS13 activity and inhibitor titers were determined using a commerical FRETS-VWF73 assay^{17 (link)} and an in-house FRETS-based assay as previously described.^{18 (link)} Plasma anti-ADAMTS13 IgG was determined by an enzyme-linked immunosorbent assay (ELISA) (Diapharma, West Chester, Ohio) in accordance with the manufacturer’s recommendations.

Staley E.M., Cao W., Pham H.P., Kim C.H., Kocher N.K., Zheng L., Gangaraju R., Lorenz R.G., Williams L.A., Marques M.B, & Zheng X.L. (2019). Clinical factors and biomarkers predict outcome in patients with immune-mediated thrombotic thrombocytopenic purpura. Haematologica, 104(1), 166-175.

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