Skyscan micro ct scanner

The mice were fed with HFD (MP Biomedicals) containing 60 kcal% calorie value, which was replaced every three days and weighted to calculate food intake. When necessary, mice were subcutaneously injected with either vehicle or LPZ (10 mg/kg) daily until the completion of the experiments, when serum, different adipose depots and liver samples were dissected and weighted for subsequent analysis. The bone and fat volume of mice was recorded using the SKyScan micro-CT scanner (Bruker). Volumetric micro-CT data were post-processed via Gaussian smoothing, and analyzed using the CTAn software (Bruker), wherein regions of interest were manually delineated. Energy expenditure was measured by the Promethion calorimetry system (Sable Systems International).

Micro-computed tomography assessment of cartilage/bone microarchitecture was carried out on some rabbit joints using a High throughput in vivo SkyScan micro-CT scanner (Bruker, Billerica, MA) with an exposure time of 640 ms/frame and the X-ray power at 39 W during an 18 min scan. 499 images were obtained at a rotation step of 0.72°. The scans were then reconstructed and analyzed using CTVox and CTAn software (Bruker, Billerica, MA). To avoid selecting the wrong defect areas, we decide to evaluate the whole joint and the bone volume fraction (BVF), which is defined as bone volume divided by tissue volume (BV/TV), for all of the samples [8 (link),28 (link)].