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P 308 akt

Manufactured by Cell Signaling Technology
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P-308-Akt is a laboratory equipment product offered by Cell Signaling Technology. It is used to detect and quantify the phosphorylation of the Akt protein at serine 308. This product can be used in various biochemical and cell-based assays to study the activity and regulation of the Akt signaling pathway.

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Lab products found in correlation

P473akt, by Cell Signaling Technology (3 mentions) Vinculin, by Merck Group (2 mentions) Antibody to vinculin, by Merck Group (1 mentions) Total akt, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) P p70s6k, by Cell Signaling Technology (1 mentions) Anti au1 antibody, by Biosynth (1 mentions) P gsk3a b, by Cell Signaling Technology (1 mentions) P mdm2, by Cell Signaling Technology (1 mentions) Mlc2v, by Synaptic Systems (1 mentions) T akt, by Cell Signaling Technology (1 mentions) T p70s6k, by Cell Signaling Technology (1 mentions) Gapdh, by Biosynth (1 mentions) Cyclin d1, by Cell Signaling Technology (1 mentions) 4e bp1, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Anti-p–Akt-308 P-AKT Akt/p-Akt

3 protocols using p 308 akt

1

Skeletal Muscle Signaling Pathway Analysis

Cited 1 time
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RT-PCR and immunoblotting were conducted as previously described.41 (link) Skeletal muscle underwent evaluation of Akt and AMPK activation using antibodies obtained from Cell Signaling Technology (Danvers, MA): p-308-Akt (#4056), p-473-Akt (#4058), total Akt (# 9272), p-172-AMPK (#2535), and GAPDH (# 51332S). Antibody to vinculin was obtained from Sigma (St. Louis, MO; SAB4200080).
Gao M.H., Giamouridis D., Lai N.C., Guo T., Xia B., Kim Y.C., Huu V.A., Skowronska-Krawczyk D., Lantier L., Bhargava R, & Hammond H.K. (2019). Urocortin 2 Gene Transfer Improves Glycemic Control and Reduces Retinopathy and Mortality in Murine Insulin Deficiency. Molecular Therapy. Methods & Clinical Development, 17, 220-233.
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2

Adenylyl Cyclase Protein Expression Analysis

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Expression of adenylyl cyclase related proteins were analyzed using
Agilent Gene Expression Microarray technique by PhalanxBio Inc. (CA, San Diego).
C1C2 protein was detected by anti-AU1 antibody (Fitzgerald, 1:2,000) or
anti-AC5/6 antibody (Santa Cruz, 1:200). Additional antibodies included: GAPDH
(Fitzgerald, 1:20,000); cMLCK (Abgent, 1:1,000); MLC2v (Synaptic Systems,
1:1,000) PKA catalytic subunit (BD Transduction, 1:1,000); p-ERK1/2, p38,
p-PKARII α and β (Santa Cruz Biotech, 1:200); PLB (Affinity
Bioreagents, 1:5,000); phospho-16-PLB (Badrilla, 1:3,000); S100A1 (Epiyomics,
1:1,000); SERCA2a antibody (Enzo, 1:1,000); phospho-αB-crystallin
(CryAB) and total CryAB antibodies (Enzo, 1:1,000); P-308-Akt, P-473-Akt, T-Akt,
p-GSK3a/b, p-MDM2, P-p70S6K, T-p70S6K, and phospho-S22/23 troponin I (TnI) (Cell
Signaling, 1:1,000 each); Vinculin (Sigma, 1:100,1000).
Gao M.H., Lai N.C., Giamouridis D., Kim Y.C., Tan Z., Guo T., Dillmann W.H., Suarez J, & Hammond H.K. (2016). Cardiac-Directed Expression of Adenylyl Cyclase Catalytic Domain Reverses Cardiac Dysfunction Caused by Sustained Beta-Adrenergic Receptor Stimulation. JACC: Basic to Translational Science, 1(7), 617-629.
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3

Comprehensive Immunohistochemistry Protein Profiling

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Vinculin (Sigma #V4505), ER antibodies: Santa Cruz cat# sc543 or Millipore #04-820), ER pS118 (Cell Signalling #2511), ER for IHC (Ventana #790-4324 [SP1]), p308-AKT (Cell Signaling #2965), p473-AKT (Cell Signaling #4060), AKT (Cell Signaling #4691), p240/244 S6 (Cell signaling #5364), p65-4EBP1 (Cell Signaling #9456), 4EBP1 (Cell Signaling #9452), cyclinD1 (Cell Signaling #2926), Rabbit IgG (Sigma, I5006). IHC: PR (1E2) Her2 (4B5) (Ventana Discovery XT). Biotinylated secondary antibodies (Vector Labs PK-6101).
Ladd B., Mazzola A.M., Bihani T., Lai Z., Bradford J., Collins M., Barry E., Goeppert A.U., Weir H.M., Hearne K., Renshaw J.G., Mohseni M., Hurt E., Jalla S., Bao H., Hollingsworth R., Reimer C., Zinda M., Fawell S, & D'Cruz C.M. (2016). Effective combination therapies in preclinical endocrine resistant breast cancer models harboring ER mutations. Oncotarget, 7(34), 54120-54136.
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