The antibodies used in this study were previously described as follow: polyclonal rabbit: α-MyoA and α-MLC1 (ref. 59 (link)), α-GAP40, α-IMC1 and α-GAP45 (ref. 9 (link)), α-MLC2 (ref. 28 (link)), α-Cpn60 (ref. 32 (link)), α-catalase60 (link), α-ARO12 (link), α-HSP70 (ref. 40 (link)); monoclonal mouse, α-ACT59 (link), α-ISP1 (ref. 61 (link)), α-Ty (BB2, ref. 62 (link)). α-SAG1, α-MIC2, α-GRA1, α-GRA3 and α-P21 are generous gifts from Dr J.-F. Dubremetz. For WB, secondary peroxidase-conjugated goat α-rabbit/mouse antibodies (Sigma) were used, as well as α-Myc (9E10, Santa Cruz Sc-40). For IFA, the secondary antibodies Alexa Fluor 488- and Alexa Fluor 594-conjugated goat α-mouse/rabbit antibodies (Life Technologies) as well as the DBA-fluorescein labelled (Reactolab FL-1031) were used. The dilutions of use are described in Supplementary Table 3 .
Secondary peroxidase conjugated goat α rabbit mouse antibodies
Manufactured by Merck Group
Secondary peroxidase-conjugated goat α-rabbit/mouse antibodies are laboratory reagents used to detect and quantify target proteins in various immunoassays. They act as secondary antibodies, binding to primary antibodies raised against rabbit or mouse antigens, and provide a means for signal amplification through the attached peroxidase enzyme.
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2 protocols using secondary peroxidase conjugated goat α rabbit mouse antibodies
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Antibody Characterization in Toxoplasma
2
Parasite Protein Immunodetection Protocols
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The following antibodies were used in this study: T34A11 mAb anti-MIC2 (Achbarou et al., 1991 (link)), rabbit anti-AMA1 (Lamarque et al., 2014 (link)), rabbit anti-GAP45, mAb anti-Myc (9E10), mAb anti-Ty1(BB2), mAb anti-GRA1 (Sibley et al., 1995 (link)), rabbit anti-MIC6 (Reiss et al., 2001 (link)), rabbit anti-MIC4 (Reiss et al., 2001 (link)), mAb T52A3 anti-ROP1 (unpublished), ARO (Mueller et al., 2013 (link)), ATrx (DeRocher et al., 2008 (link)), TLN1 (Hajagos et al., 2012 (link)), SAG1/ROP1/ROP2-4/ROP7/ROP13/RON9 (gift from Dr J-F Dubremetz), M2AP/proM2AP/CPL (gift from V. B. Carruthers), MIC3 T82C10 (Achbarou et al., 1991 (link)), MIC6 (Meissner et al., 2002 (link)), PLP1 (Roiko and Carruthers, 2013 (link)), MIC4 (Meissner et al., 2002 (link)), PfSUB1 (a gift from Michael Blackman), Cpn60 (Agrawal et al., 2009 (link)), RON2/RON4 (gift from M. Lebrun), MIC8 (EGF-N-term) (Meissner et al., 2002 (link)).
For western blot analyses, secondary peroxidase conjugated goat α-rabbit/mouse antibodies (Sigma) were used. For immunofluorescence analyses, the secondary antibodies Alexa Fluor 488- and Alexa Fluor 594-conjugated goat α-mouse/rabbit antibodies (Life Technologies) were used.
For western blot analyses, secondary peroxidase conjugated goat α-rabbit/mouse antibodies (Sigma) were used. For immunofluorescence analyses, the secondary antibodies Alexa Fluor 488- and Alexa Fluor 594-conjugated goat α-mouse/rabbit antibodies (Life Technologies) were used.
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