Goat anti rabbit or mouse hrp conjugated igg

Manufactured by Cell Signaling Technology

Sourced in Canada

Goat anti-rabbit or mouse HRP-conjugated IgG is a secondary antibody used for detection in Western blotting and immunohistochemistry. It is conjugated to horseradish peroxidase (HRP), which allows for chemiluminescent or colorimetric detection of the target protein.

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Lab products found in correlation

Mouse anti β actin, by Cell Signaling Technology (3 mentions) Rabbit anti bip, by Abcam (2 mentions) Stf 083030, by Santa Cruz Biotechnology (2 mentions) Rabbit anti il 1β, by Santa Cruz Biotechnology (1 mentions) Rabbit anti il 18, by Abcam (1 mentions) Rabbit anti caspase 1, by Cell Signaling Technology (1 mentions) Rat anti mlkl, by Merck Group (1 mentions) Alexa fluor 594 donkey anti mouse igg, by Thermo Fisher Scientific (1 mentions) Rabbit anti p erk, by Cell Signaling Technology (1 mentions) Rabbit anti nlrp3, by Abcam (1 mentions) Resveratrol, by Merck Group (1 mentions) Rabbit anti ubiquitin, by Cell Signaling Technology (1 mentions) Rabbit anti pdi, by Abcam (1 mentions) Rabbit anti albumin, by Abcam (1 mentions) Rabbit anti caspase 3, by Cell Signaling Technology (1 mentions) Alexa fluor 488 donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Ab201061, by Abcam (1 mentions) A0002, by Agilent Technologies (1 mentions)

3 protocols using goat anti rabbit or mouse hrp conjugated igg

Investigating Cell Signaling Pathways

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The following antibodies were used: rabbit anti-TXNIP (Cell Signaling, #14715), rabbit anti- PERK (Cell Signaling, #5683), rabbit anti-IL-18 (Abcam, ab68435), rabbit anti-NLRP3 (Abcam, ab214185), rabbit anti-IL-1β (Santa Cruz, sc-7884), rabbit anti-caspase-1 (Cell Signaling, #2225), mouse anti-Gasdermin D (Abcam, ab57785), mouse anti-β-actin (Cell Signaling), rabbit anti-BiP (Abcam, ab21685), rabbit anti-IRE1a (Abcam, ab48187), rabbit anti-phospho-MLKL (Ser358) (Cell Signaling, D6H3V), rat anti-MLKL (Millipore, MABC604), mouse anti-ASC (B-3) (Santa Cruz, sc-514414), rabbit anti-cleaved gasdermin D (Asp275) (Cell Signaling, #36425), goat anti-rabbit or mouse HRP-conjugated IgG (Cell Signaling), Alexa Fluor 488 donkey anti-rabbit or mouse IgG (Molecular Probes, A-21206), and Alexa Fluor 594 donkey anti-mouse IgG (Molecular Probes, A-21203). The following reagents were used: STF 083030 (IRE1a inhibitor, Santa Cruz, CAS 307543-71-1); resveratrol (TXNIP inhibitor, Sigma, R5010-100MG); PERK inhibitor II (Sigma, 5046510001); NLRP3 inhibitor (Sigma, 5381200001).

Cheng S.B., Nakashima A., Huber W.J., Davis S., Banerjee S., Huang Z., Saito S., Sadovsky Y, & Sharma S. (2019). Pyroptosis is a critical inflammatory pathway in the placenta from early onset preeclampsia and in human trophoblasts exposed to hypoxia and endoplasmic reticulum stressors. Cell Death & Disease, 10(12), 927.

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Investigating ER Stress Pathways

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Commercial antibodies included: rabbit anti-human transthyretin antibody (A0002, Agilent Dako, Santa Clara, CA), rabbit anti-BiP (Abcam, Cambridge, MA), rabbit anti-pIRE1a (Abcam, Cambridge, MA), rabbit anti-Ero-1 (Cell Signaling Technology, MA), rabbit anti-PDI (Abcam, Cambridge, MA), rabbit anit-LAMP 1 (Abcam, Cambridge, MA), rabbit anti-albumin (Abcam, Cambridge, MA) rabbit anti-ubiquitin (Santa Cruz, CA), rabbit anti-caspase-3 (Cell Signaling Technology, MA), rabbit anti-SQSMT1/p62 (MBL), mouse anti-β-actin (Cell Signaling Technology, MA), goat anti-rabbit or mouse HRP-conjugated IgG (Cell Signaling Technology, MA), donkey anti-rabbit Alexa 488 or 594 (ThermoFisher Scientific, MA). STF 083030 (an IRE1a inhibitor) was purchased from, Santa Cruz, CAS (307543-71-1)

Cheng S., Huang Z., Banerjee S., Jash S., Buxbaum J.N, & Sharma S. (2022). Evidence from human placenta, ER-stressed trophoblasts and transgenic mice links transthyretin proteinopathy to preeclampsia. Hypertension (Dallas, Tex. : 1979), 79(8), 1738-1754.

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Antibody Panel for Protein Detection

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The following antibodies were used in immunostaining or western blotting: rabbit anti-TTR (A0002, Agilent Dako, Santa Clara, CA), rabbit anti-beta Amyloid 1–42 antibody [mOC98] (ab201061, abcam, Cambridge, MA), rabbit anti-α-syn (ab131508, abcam, Cambridge, MA), SERPINA1 (ARP59239_P050, Aviva Systems Biology, San Diego, CA), mouse anti-β-actin (Cell Signaling Technology), goat anti-rabbit or mouse HRP-conjugated IgG (Cell Signaling Technology), and Alexa Fluor 488 donkey anti-rabbit IgG (Molecular Probes, A-21206).

Cheng S., Banerjee S., Daiello L.A., Nakashima A., Jash S., Huang Z., Drake J.D., Ernerudh J., Berg G., Padbury J., Saito S., Ott B.R, & Sharma S. (2021). Novel blood test for early biomarkers of preeclampsia and Alzheimer’s disease. Scientific Reports, 11, 15934.

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