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Phosstop easy

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PhosSTOP EASY is a ready-to-use phosphatase inhibitor cocktail. It is designed to inhibit serine/threonine and tyrosine phosphatases in cell and tissue extracts.

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Lab products found in correlation

Protease inhibitor cocktail, by Roche (1 mentions) β mercaptoethanol, by Nacalai Tesque (1 mentions)

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PhosSTOP (1722 citations)

2 protocols using phosstop easy

1

Protein Extraction from Plant Tissues

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Liquid nitrogen powders of leaves and/or sprouts of Arabidopsis, rice, wheat, broccoli, soy, tomato, fern, and Selaginella moellendorfii were lysed in Plant Lysis Buffer: 50 mM Tris-HCl pH 7.5 at room temperature, 150 mM NaCl, 5 mM EGTA, 10% glycerol, 1% NP40, 1 mM DTT, plant-specific protease inhibitors (Sigma) and phosphatase inhibitors (PhosSTOP EASY, Roche).
McWhite C.D., Papoulas O., Drew K., Cox R.M., June V., Dong O.X., Kwon T., Wan C., Salmi M.L., Roux SJ J.r., Browning K.S., Chen Z.J., Ronald P.C, & Marcotte E.M. (2020). A pan-plant protein complex map reveals deep conservation and novel assemblies. Cell, 181(2), 460-474.e14.
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2

Whole Cell Lysate Preparation and Immunoblotting

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Whole cell lysates from 5.0 × 106 cells, prepared using an SDS-based buffer (5 mM EDTA, 1% SDS) supplemented with Protease inhibitor cocktail (Roche) and PhosSTOP EASY (Roche), were mixed with an equal volume of twofold concentrated sample buffer (Bio-Rad Laboratories) containing β-mercaptoethanol (Nacalai Tesque), and were treated for 5 min at 100°C. Immunoblot analysis was performed as described previously using a mouse anti-FLAG antibody (Millipore, clone JBW301) or a mouse anti-α-tubulin monoclonal antibody (AA13, Funakoshi).
Yamazaki H., Shirakawa K., Matsumoto T., Kazuma Y., Matsui H., Horisawa Y., Stanford E., Sarca A.D., Shirakawa R., Shindo K, & Takaori-Kondo A. (2020). APOBEC3B reporter myeloma cell lines identify DNA damage response pathways leading to APOBEC3B expression. PLoS ONE, 15(1), e0223463.
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