Groups of 20 flies were exposed to 30 min of 20% ethanol to avoid sedation, then frozen in liquid nitrogen either immediately for “absorption” samples, or 30 min later for “metabolism” samples. After homogenization in 50 mM Tris-HCl, pH 7.5, ethanol concentrations were measured using the NAD-ADH Reagent kit following the manufacturer’s protocol (Sigma-Aldrich, N7160). The 340 nm spectrophotometric absorbance values were converted to mM and adjusted by the estimated 1 μL volume of an average fly to calculate ethanol concentrations.
Nad adh reagent kit
Manufactured by Merck Group
Sourced in Germany
The NAD-ADH Reagent kit is a laboratory product designed to detect and measure the presence of nicotinamide adenine dinucleotide (NAD) and alcohol dehydrogenase (ADH) in samples. The kit provides the necessary reagents and components to perform these analyses in a controlled laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using nad adh reagent kit
1
Determining Fly Ethanol Absorption
2
Alcohol Pharmacokinetics in Animal Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In a separate group of animals, we determined blood alcohol levels (BALs) after an IP injection of 0.6 and 1.2 g/kg alcohol. Thirty minutes after injection, blood samples were collected from the lateral tail vein in EDTA-coated capillary tubes (Sarstedt, Numbrecht, Germany) and immediately stored on ice. In addition, to explore the metabolism of alcohol over time, animals were treated with 0.6 g/kg for blood sampling at 5, 10, 15, 30, 60, and 120 min after injection. Blood samples were spun at 3000 rpm for 20 min (at 4 °C) and plasma was stored at −20 °C until blood alcohol analysis. BALs (mg/dl) were determined using an NAD-ADH reagent kit (Sigma-Aldrich, Schnelldorf, Germany) and a standard curve for quantitation.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!