For measuring luminescence from HEK cells, reconstituted and diluted CTZ was added to the culture medium immediately before imaging to the final concentration indicated in the experiment. Luminescence was measured using an IVIS Lumina LT In Vivo Imaging System (PerkinElmer, Waltham, MA) with Living Image 4.5.2 software (RRID:SCR_014247). Images were displayed as a pseudo-color photon counted image. Regions of interest were defined using an automatic intensity contour procedure to identify bioluminescent signals with intensities significantly greater than background. The sum of the photon counts in these regions was then calculated.
Ivis lumina lt in vivo imaging system
Manufactured by PerkinElmer
Sourced in United States
The IVIS Lumina LT In Vivo Imaging System is a bioluminescence and fluorescence imaging platform designed for preclinical research. It enables the detection and quantification of light-emitting reporter molecules, such as luciferase and fluorescent proteins, within living subjects.
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6 protocols using ivis lumina lt in vivo imaging system
1
Quantifying HEK Cell Luminescence
2
Monitoring Cell Proliferation and Metastasis in 4T1 CSC Mice
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Organ imaging was used to monitor cell proliferation and metastasis in RFP expressing 4T1 derived CSCs injected mice of different treatment groups. Tumor tissue and lung were collected for organ imaging and data were acquired by IVIS® Lumina LT In Vivo Imaging System, PerkinElmer.
3
Bioluminescence Imaging of Cultured Neurons
4
Bioluminescent Imaging of Anesthetized Animals
5
In Vivo Bioluminescence Imaging
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Bioluminescence imaging was acquired using the IVIS Lumina LT In Vivo Imaging System (Perkin Elmer, Boston, MA, USA) with Living Image version 4.5.5 software (Perkin Elmer, Boston, MA, USA). Mice were infused by intraperitoneal injection with 150 mg/kg D-luciferin potassium salt (Wako, Osaka, Japan) suspended in 200 μL PBS. 5–15 minutes later, mice were imaged under 2% isoflurane anesthesia. Images were acquired on a 25-cm field of view at medium binning level for within 1 min exposure time.
6
Evaluating Dara's Impact on GVL Effect
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We also evaluated whether Dara impacted on the GVL effect when it acted as an immunomodulatory factor. Nalm6 cells were engineered to express the green fluorescent protein (GFP) and luciferase reporter genes (Nalm6.LucGFP cells) for longitudinal tumor load monitoring by bioluminescence imaging. Female NSG mice at 8–12 weeks of age were irradiated (1.8 Gy) at day ˗1 and received intravenous injection of 1×105 Nalm6.LucGFP cells or 1× 105 Nalm6.LucGFP cells plus 1×107 hPBMCs through the tail vein on day 0. Dara (5 mg/kg) or human IgG control antibody were intraperitoneally injected once a week for two weeks post-transplantation. Each experimental group included ten mice. Mice were assessed for survival every other day. Leukemia cell growth was evaluated weekly with bioluminescence imaging using the IVIS® Lumina LT in vivo Imaging System (Perkin-Elmer). Imaging data were analyzed and quantified with Living Image 3.0 Software (Calipers).
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