Spss v 19.0 for windows

All analyses were performed using SPSS v. 19.0 for Windows (IBM Corp., Armonk, NY, USA) and GraphPad Prism v. 5.0 for Windows (GraphPad Software Inc., La Jolla, CA, USA). For comparisons of two treatment groups, a Student's t-test was used. For comparisons of three or more groups, one-way analysis of variance was followed by the Bonferroni post hoc test for comparison of two selected treatment groups; the Dunnett's post hoc test was used for comparisons of the other treatment groups with the corresponding controls. The Pearson's correlation analysis was used to determine the r-value. Associations between miR-577 expression and clinicopathological characteristics were assessed using chi-squared test. Data from at least three independent experiments are presented as the means ± standard deviation, or medians with ranges. P<0.05 was considered to indicate a statistically significant difference.

Where appropriate, all the results were obtained from triplicate experiments performed in a parallel manner, and depicted as the mean ± SD. The tumor volumes and weights of each group were compared by one-way ANOVA, and the survival curves were estimated according to the Kaplan–Meier method. The levels of the P values for comparison between all groups were determined by a 2-tailed Mann–Whitney test, and a probability value of ≤0.05 was considered to be statistically significant. Statistical analyses were performed with the statistical software system of SPSS v19.0 for windows (IBM, Armonk, NY).

All data are presented as the mean ± SD. Statistical differences between groups was performed with one-way analysis of variance using the post hoc Tukey’s test. Data were analyzed using SPSS v.19.0 for Windows (IBM, Armonk, NY, USA). P value less than 0.05 was considered statistically significant.

Where appropriate, all the results were obtained from triplicate experiments performed in a parallel manner, and depicted as the mean ± standard deviation. The tumor volumes and weights of each group were compared by one-way ANOVA (analysis of variance), and the survival curves were estimated according to the Kaplan–Meier method. The levels of P-values for comparison between all groups were determined by 2-tailed Mann–Whitney test, and a probability value of ≤0.05 was considered to be statistically significant. Statistical analyses were performed with the statistical software system of SPSS v19.0 for windows (IBM, Armonk, NY, USA).

The patient’s clinicopathological characteristics were summarized descriptively and tested using two-sample Student t-tests (continuous variables) and Pearson’s Chi-square tests (categorical variables). Wilcoxon–Mann–Whitney tests were used to analyze the TNM stage and histological grade. The DFS and DSS of subgroups were compared to determine survival outcomes using Kaplan–Meier curves and then further analyzed with the log-rank test to observe the significance. All possible prognostic factors were determined using a univariate Cox proportional hazards model; subsequently, the meaningful prognostic factors (defined as those with P<0.05 in univariate analysis) were further assessed in a multivariate analysis. Interaction between EYA2 expression status and chemotherapy was evaluated using Cox proportional hazards models with a 2×2 factorial design.21 (link) All statistical analyses were conducted using SPSS V.19.0 for Windows (IBM Corporation, Armonk, NY, USA). Significance was set at P<0.05 for two-sided tests.