Insulin

Manufactured by Calbiotech

Sourced in China, France

Insulin is a laboratory equipment used to measure and analyze insulin levels in biological samples. It serves as a core tool for researchers and clinicians to assess insulin production, secretion, and metabolism in the body.

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Lab products found in correlation

Testosterone, by Calbiotech (3 mentions) Cortisol, by Calbiotech (3 mentions) Glucagon, by BioAssay Systems (3 mentions) Dtx 880 multimode detector, by Beckman Coulter (3 mentions) Oestrogen, by Calbiotech (2 mentions) Tnf α, by Diaclone (2 mentions) Corticosterone, by Demeditec Diagnostics (1 mentions)

7 protocols using insulin

Glucose and Hormone Assay Protocol

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Blood glucose was estimated by glucose oxidase method using glucometer [26 (link)].
Plasma insulin, cortisol, testosterone, oestrogen (Calbiotech Inc., 1935 Cordell Ct., El Cajon, CA 92020) and glucagon (Bioassay Technology Laboratory, Yangpu Dist, Shanghai, China) were assayed spectrophotometrically (Beckman Coulter DTX 880 Multimode Detector) following the kit manufacturers’ procedure.

Alagbonsi A.I., Salman T.M., Sulaiman S.O., Adedini K.A, & Kebu S. (2021). Possible mechanisms of the hypoglycaemic effect of artesunate: Gender implication. Metabolism Open, 10, 100087.

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Evaluating Bone Remodeling Biomarkers

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TNF-α (Diaclone, France), Insulin (Calbiotech, USA), RANK (Elabscience, USA), RANKL (Elabscience, USA) and OPG (Fine test, China) were estimated by an enzyme-linked immunosorbent assay based on the principle of sandwich ELISA and the mean value was determined from the standard dose–response curves. HOMA-IR was calculated using formula HOMA-IR = Glucose X Insulin/405^{29 (link)}. The inter-assay coefficients of variation for ELISA parameters for RANKL, RANK, TNF-α, OPG and Insulin were < 6%, < 7%, < 11%, < 5.5% and < 6.1% respectively. The intra-assay of coefficients of variation for ELISA parameters for RANKL, RANK, TNF-α, OPG and Insulin were < 6%, < 5.5%, < 3.2%, < 5.5% and < 3.5% respectively.

Sailaja A.N., Nanda N., Suryanarayana B.S, & Pal G.K. (2023). Association of rs2073618 polymorphism and osteoprotegerin levels with hypertension and cardiovascular risks in patients with type 2 diabetes mellitus. Scientific Reports, 13, 17451.

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Quantifying Hormonal Biomarkers

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Insulin (product code: IN374S) and cortisol (product code: CO103S) kits were purchased from the Calbiotech Inc., 1935 CordellCt., El Cajon, CA 92020. Glucagon (product code: E1066Ra) and adrenaline (product code: E0545Ra) kits were purchased from the Bioassay Technology Laboratory, 1713 Junjiang International Bldg, 218 Ningguo Rd, Yangpu Dist, Shanghai, China). All assays were done spectrophotometrically (Beckman Coulter DTX 880 Multimode Detector) following manufacturers’ procedures.

Salman T.M., Alagbonsi I.A, & Sulaiman S.O. (2020). Counter-regulatory responses to Telfairia occidentalis-induced hypoglycaemia. Metabolism Open, 8, 100065.

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ELISA Measurement of Serum Biomarkers

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Serum leptin (Diagnostics Biochem Canada Inc. Canada), insulin (Cal biotech, California) and TNF-α (Orgenium Laboratories Business Unit, Finland) were measured using Sandwich ELISA technique according to the manufacturer’s instructions.

Nalini D., Karthick R., Shirin V., Manohar G, & Malathi R. (2015). “Role of the adipocyte hormone leptin in cardiovascular diseases – a study from Chennai based Population”. Thrombosis Journal, 13, 12.

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Quantification of Inflammatory and Metabolic Markers

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TNF-α (Diaclone, France), insulin (Calbiotech, USA), and OPG (Fine test, China) were estimated by an enzyme-linked immunosorbent assay (ELISA). In the method of ELISA for OPG, the polyclonal goat anti-human OPG antibody binds to human endogenous OPG. Thus, the monomeric and dimeric OPG form a complex with the antibody. The washing step removes the nonspecific bound materials, while a second antibody complexed with streptavidin-HRP detects this complex, which follows the principle of sandwich ELISA. The concentration of OPG is directly determined by the standard dose-response curve. HOMA-IR was calculated using formula. HOMA-IR = Glucose X insulin / 405 [30 (link)]. The inter-assay and intra-assay coefficients of variation for ELISA parameters were less than 3.5 and 6.1%, respectively.

Sailaja A.N., Nanda N., Suryanarayana B.S, & Pal G.K. (2022). Hypertension attenuates the link of osteoprotegerin to reduced baroreflex sensitivity in type 2 diabetes mellitus patients on oral antidiabetic and antihypertensive therapy – a cross sectional study. BMC Endocrine Disorders, 22, 226.

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Biochemical Analyte Measurements in Plasma

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Blood glucose was estimated by glucose oxidase method using glucometer (26).
Plasma insulin, cortisol, testosterone, oestrogen (Calbiotech Inc., 1935 Cordell Ct., El Cajon, CA 92020) and glucagon (Bioassay Technology Laboratory, Yangpu Dist, Shanghai, China) were assayed spectrophotometrically (Beckman Coulter DTX 880 Multimode Detector) following the kit manufacturers' procedure.

Alagbonsi A.I., Salman T.M., Sulaiman S.O., Kafayat A., & Kebu S. (2020). Possible mechanisms of the hypoglycaemic effect of artesunate: Gender implication.

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Endocrine Biomarkers Quantification

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Serum concentrations of estradiol, testosterone, insulin (Calbiotech, Spring Valley, CA, USA) and corticosterone (Demeditec diagnostics GmbH, Germany) were estimated using ELISA kits and procedures provided by the respective manufacturer.

Divyashree S, & Yajurvedi H.N. (2016). Long-term chronic stress exposure induces PCO phenotype in rat. Reproduction (Cambridge, England), 152(6).

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