Purmorphomine

Manufactured by Bio-Techne

Sourced in United States

Purmorphomine is a small molecule compound that functions as a selective agonist for the Sonic Hedgehog (Shh) signaling pathway. It is used for in vitro cell culture research applications.

Automatically generated - may contain errors

Lab products found in correlation

Recombinant mouse shh n terminus, by R&D Systems (2 mentions) Pd173074, by Bio-Techne (2 mentions) Api 2, by Bio-Techne (2 mentions) Ly294002, by Merck Group (2 mentions) Fibroblast growth factor 2 (fgf2), by R&D Systems (2 mentions) Rapamycin, by Selleck Chemicals (1 mentions)

2 protocols using purmorphomine

Modulating Signaling Pathways in Cells

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Recombinant mouse Shh N-terminus (1 μg/mL, R&D systems, Minneapolis, MN) or Purmorphomine (2 μM, Tocris Bioscience, 4551) were used to activate Hh signaling. FGF2 (10 ng/mL, R&D systems, Minneapolis, MN) was used to activate FGF signaling. BMP2 (100 ng/mL) was used to activate BMP2 signaling. PD173074 (1 μM, Tocris 3044) was used to inhibit FGFR. LY294002 (15 μM, Sigma, L9908) and API-2 (1 μM, Tocris 2151), were employed to inhibit PI3K and AKT respectively.

Yuan X., Liu M., Cao X, & Yang S. (2019). Ciliary IFT80 regulates dental pulp stem cells differentiation by FGF/FGFR1 and Hh/BMP2 signaling. International Journal of Biological Sciences, 15(10), 2087-2099.

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Hh and FGF Signaling Modulation

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Recombinant mouse Shh N-terminus (1 μg/mL, R&D Systems, Minneapolis, MN, USA) or purmorphomine (2 μM, Tocris Bioscience, 4551) was used to activate Hh signaling. FGF2 (10 ng/mL, R&D Systems, Minneapolis, MN, USA) was used to activate FGF signaling. PD173074 (1 μM, Tocris, 3044) was used to inhibit FGFR. LY294002 (15 μM, Sigma, L9908), API-2 (1 μM, Tocris, 2151), rapamycin (0.5 μM, Selleckchem, S1039) were employed to inhibit PI3K, AKT, and mTOR, respectively.

Yuan X., Cao X, & Yang S. (2019). IFT80 is required for stem cell proliferation, differentiation, and odontoblast polarization during tooth development. Cell Death & Disease, 10(2), 63.

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