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Cd3 buv805

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The CD3 BUV805 is a fluorescent-labeled antibody that binds to the CD3 antigen, which is a component of the T cell receptor complex. It is designed for use in flow cytometry applications to identify and analyze T cells.

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Lab products found in correlation

Cd56 buv737, by BD (2 mentions) Cd56 pe dazzle 594, by BioLegend (2 mentions) Cd137 pe cyanine7, by BioLegend (2 mentions) Cd57 bv605, by BioLegend (2 mentions) Cd7 bv786, by BioLegend (2 mentions) Cd16 buv496, by BD (2 mentions) Cd337 nkp30 bv421, by BD (2 mentions) Cd159a nkg2a biotin pe vio770, by Miltenyi Biotec (2 mentions) Cd159c nkg2c pe, by Miltenyi Biotec (2 mentions) Cd337 nkp30 efluor 450, by Thermo Fisher Scientific (2 mentions) Cd14 apc efluor 780, by Thermo Fisher Scientific (2 mentions) Streptavidin buv395, by BD (2 mentions) Facsaria 2, by BD (2 mentions) Prism v8, by GraphPad (2 mentions) Cd3 apc efluor780, by Thermo Fisher Scientific (2 mentions) Cd19 apc efluor780, by Thermo Fisher Scientific (2 mentions) Zombie aqua fixable viability kit, by BioLegend (2 mentions) Lsrfortessa, by BD (2 mentions) Fixable viability dye efluor 780, by Thermo Fisher Scientific (2 mentions) Cd16 bv650, by BD (1 mentions)

Spelling variants of this product

BUV805 (6 citations) Anti-CD3-BUV805 (2 citations)

4 protocols using cd3 buv805

1

Multiparametric Flow Cytometry of tPCLS

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Single‐cell suspensions of tPCLS were generated as described above. Single‐cell suspensions were blocked with FcR blocking reagent (Miltenyi Biotec) in 0.5% PBS‐BSA for 20 min, stained with fluorochrome‐conjugated antibodies, and analyzed on a FACSSymphony A5SE flow cytometer (BD Biosciences). Live single cells were identified by FSC and SSC characteristics. The data were analyzed using FlowJo V10 (TreeStar). All antibodies and secondary reagents were titrated to determine optimal concentrations. Comp‐Beads (BD) were used for single‐color compensation to create multicolor compensation matrices. For gating, fluorescence minus one control was used. The instrument calibration was controlled daily using Cytometer Setup and Tracking Beads (BD Biosciences). The following antibodies were used: CD3‐BUV805 (#612896, BD Biosciences), CD4‐BB630 (#562316, BD Biosciences), CD8‐BV650 (#743067, BD Biosciences), CD14‐PerCP‐Cy5.5 (#561116, BD Biosciences), CD15‐BUV805 (#742057, BD Biosciences), CD16‐BV650 (#563692, BD Biosciences), CD19‐APC‐H7 (#560252, BD Biosciences), CD25‐PE‐Cy7 (#557741, BD Biosciences), CD33‐BV510 (#563257, BD Biosciences), CD45‐AF700 (#368514, BD Biosciences), CD80‐BV711 (#740801, BD Biosciences), CD206‐PE/Cy7 (#321124, BioLegend), CD326‐FITC (#324203, BioLegend), HLA‐DR‐APC/Fire750 (#307658, BioLegend), MerTK‐BV421 (#367603, BioLegend).
Karger A., Mansouri S., Leisegang M.S., Weigert A., Günther S., Kuenne C., Wittig I., Zukunft S., Klatt S., Aliraj B., Klotz L.V., Winter H., Mahavadi P., Fleming I., Ruppert C., Witte B., Alkoudmani I., Gattenlöhner S., Grimminger F., Seeger W., Pullamsetti S.S, & Savai R. (2023). ADPGK‐AS1 long noncoding RNA switches macrophage metabolic and phenotypic state to promote lung cancer growth. The EMBO Journal, 42(18), e111620.
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2

Multi-Dimensional Immune Profiling

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For protein expression analysis following isolation cells were collected and stained with the following antibodies for surface protein expression: CD3 APC-Cy7 (BioLegend), CD4 AF-700 (BioLegend), CD8 PE-Cy7 (BioLegend), CD45RA BV-605 (BioLegend), CD27 AF-488 (BioLegend), CD8 BUV-496 (BD Biosciences), CD3 BUV-805 (BD Biosciences), CD4 BV-750 (BD Biosciences), CD45RA BUV-395 (BD Biosciences), and CCR7 BV-786 (BD Biosciences). Dead cells were excluded from the analysis by using Zombie-UV (Biolegend). Doublets and double-positive CD4/CD8 cells were removed through sequential gating. Flow cytometry acquisition was done using the BD LSRII with BD FACSDiva. Data was analyzed by FlowJo 10.1r7 and GraphPad Prism 9.
Bukhari S., Henick B.S., Winchester R.J., Lerrer S., Adam K., Gartshteyn Y., Maniar R., Lin Z., Khodadadi-Jamayran A., Tsirigos A., Salvatore M.M., Lagos G.G., Reiner S.L., Dallos M.C., Mathew M., Rizvi N.A, & Mor A. (2022). Single-cell RNA sequencing reveals distinct T cell populations in immune-related adverse events of checkpoint inhibitors. Cell Reports Medicine, 4(1), 100868.
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3

Multiparametric Flow Cytometry of Human NK Cells

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Cell suspensions were stained with combinations of the following fluorochrome-conjugated antibodies following established guidelines57 (link): CD56 PE/Dazzle 594 (1:200), CD137 PE/cyanine7 (1:50) and CD57 BV605 (1:25; all BioLegend); CD7 BV786 (1:25), CD56 BUV737 (1:50), CD3 BUV805 (1:50), streptavidin BUV395 (1:100), CD16 BUV496 (1:50) and CD337 (NKp30) BV421 (1:25; all BD Biosciences); CD159a (NKG2A) biotin/PE-Vio770 (both 1:50) and CD159c (NKG2C) PE (1:100; all REAfinity, Miltenyi Biotec); CD337 (NKp30) eFluor 450 (1:25), CD3 APC-eFluor 780 (1:50), CD14 APC-eFluor 780 (1:50) and CD19 APC-eFluor 780 (1:50; all Thermo Fisher) and anti-FcεRI, γ subunit-FITC (1:50; Merck). Dead cells were excluded using Fixable Viability Dye eFluor 780 (Thermo Fisher) or a Zombie Aqua Fixable Viability kit (BioLegend). Data were acquired on an LSR Fortessa (BD Biosciences). Cell sorting was performed on a FACSAria II (BD Biosciences). FlowJo v10 was used for analysis of flow cytometry data, and GraphPad Prism v8.4.3 was used for statistical analysis.
Rückert T., Lareau C.A., Mashreghi M.F., Ludwig L.S, & Romagnani C. (2022). Clonal expansion and epigenetic inheritance of long-lasting NK cell memory. Nature Immunology, 23(11), 1551-1563.
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4

Multiparametric Flow Cytometry of Human NK Cells

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Cell suspensions were stained with combinations of the following fluorochrome-conjugated antibodies following established guidelines57 (link): CD56 PE/Dazzle 594 (1:200), CD137 PE/cyanine7 (1:50) and CD57 BV605 (1:25; all BioLegend); CD7 BV786 (1:25), CD56 BUV737 (1:50), CD3 BUV805 (1:50), streptavidin BUV395 (1:100), CD16 BUV496 (1:50) and CD337 (NKp30) BV421 (1:25; all BD Biosciences); CD159a (NKG2A) biotin/PE-Vio770 (both 1:50) and CD159c (NKG2C) PE (1:100; all REAfinity, Miltenyi Biotec); CD337 (NKp30) eFluor 450 (1:25), CD3 APC-eFluor 780 (1:50), CD14 APC-eFluor 780 (1:50) and CD19 APC-eFluor 780 (1:50; all Thermo Fisher) and anti-FcεRI, γ subunit-FITC (1:50; Merck). Dead cells were excluded using Fixable Viability Dye eFluor 780 (Thermo Fisher) or a Zombie Aqua Fixable Viability kit (BioLegend). Data were acquired on an LSR Fortessa (BD Biosciences). Cell sorting was performed on a FACSAria II (BD Biosciences). FlowJo v10 was used for analysis of flow cytometry data, and GraphPad Prism v8.4.3 was used for statistical analysis.
Rückert T., Lareau C.A., Mashreghi M.F., Ludwig L.S, & Romagnani C. (2022). Clonal expansion and epigenetic inheritance of long-lasting NK cell memory. Nature Immunology, 23(11), 1551-1563.
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