Anti mfg e8 sc 271574

The primary antibodies used in our study, anti-α-SMA (ab5694), anti-Fibronectin (ab2413), anti-CD34 (ab81289), and anti-CD44 (ab189524) were obtained from Abcam (Cambridge, UK). Anti-E-cadherin (20874-1-AP), anti-SOD1 (10269-1-AP), anti-Catalase (21260-1-AP), anti-cleaved caspase-3 (19677-1-AP), and anti-PARP1(13371-1-AP) were obtained from Proteintech (Wuhan, China). Anti-RhoA (sc-418), anti-ROCK1 (sc-17794), and anti-MFG-E8 (sc-271574) were purchased from Santa Cruz Technology (California, USA

The primary antibodies used in our study, anti-α-SMA (ab5694), anti-Fibronectin (ab2413), anti-CD34 (ab81289), and anti-CD44 (ab189524) were obtained from Abcam (Cambridge, UK). Anti-E-cadherin (20874-1-AP), anti-SOD1 (10269-1-AP), anti-Catalase (21260-1-AP), anti-cleaved caspase-3 (19677-1-AP), and anti-PARP1(13371-1-AP) were obtained from Proteintech (Wuhan, China). Anti-RhoA (sc-418), anti-ROCK1 (sc-17794), and anti-MFG-E8 (sc-271574) were purchased from Santa Cruz Technology (California, USA). Anti-p-MYPT1 (4563T) was purchased from Cell Signaling Technology (Massachusetts, USA). Anti-CD11b (201807), anti-CD90 (206105) were purchased from Biolegend (California, USA). GAPDH (AG019) and Reactive Oxygen Species Assay Kit were obtained from Beyotime (Shanghai, China). Y-27632 (S104921) was purchased from Selleck (Texas, USA). Recombinant Human TGF-β1 (100-21) was purchased from Peprotech (New Jersey, USA). Mesenchymal Stem Cell Adipogenic Differentiation Medium (RASMX-90031), Mesenchymal Stem Cell Chondrogenic Differentiation Medium (RASMX-90041), and Mesenchymal Stem Cell Osteogenic Differentiation Medium (RASTA-90021) were purchased from Cyagen (California, USA). RhoA Activation Assay Combo Biochem Kit (BK030) was purchased from Cytoskeleton (California, USA).