HGC-27 cells were seeded on a 6-well plate. After reaching 80% confluency, HGC-27 cells were transfected with SNHG15 plasmid, SNH15 siRNA, or miR-141 mimic by Lipofectamine 2000 according to the manufacturer’s protocol. After 48 h, cells were collected. After wash with PBS for 3 times, cells were blocked with 0.1% BSA for 30 min, stained with FAM-labeled PD-L1 antibody (BioLegend, USA) (1:200) for another 30 min. After wash with PBS for 3 times, FAM-positive cells were then analyzed by flow cytometry.
Fam labeled pd l1 antibody
Manufactured by BioLegend
Sourced in United States
The FAM-labeled PD-L1 antibody is a fluorescently-labeled monoclonal antibody that specifically binds to the programmed death-ligand 1 (PD-L1) protein. The fluorescent FAM (6-carboxyfluorescein) label allows for the detection and visualization of PD-L1 expression in various experimental applications.
Automatically generated - may contain errors
2 protocols using fam labeled pd l1 antibody
1
PD-L1 Surface Expression in HGC-27 Cells
2
Cytotoxicity and Immune Response Profiling
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The quantified levels of IFN-γ, TNF-α, Granzyme B secreted by co-cultured activated CD8+ T cells were detected using ELISA kits, including Human TNF-α ELISA Kit, IFN-γ ELISA Kit (MultiSciences, China) and Human Granzyme B ELISA Kit (Beyotime Institute of Biotechnology) according to the manufacturer’s protocol. The supernatants were collected and detected by 450 nm Optical densities using microplate reader (Bio-Tek). For the surface PD-L1, ~ 80% confluency BC cells were stained by FAM-labeled PD-L1 antibody (BioLegend, USA). After washing by PBS three times, FAM-positive cells were detected by flow cytometry. The release of lactate dehydrogenase (LDH) in the supernatants was detected on cell lysis using the Cytotoxicity Detection Kit PLUS (Sigma-Aldrich) according to the manufacturer’s protocol.
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