Sorbitane monostearate span 60

Acrylic acid (anhydrous 99%), N,N′-methylenebisacrylamide (99%), N,N,N′,N′-tetramethylethylenediamine (TEMED) (reagent plus 99%), ammonium persulfate (≥98%), sorbitane monostearate (Span 60^®), sodium chloride, sodium phosphate monobasic (ReagentPlus^® ≥ 99%), sodium phosphate dibasic (ReagentPlus^® ≥ 99%), amitriptyline hydrochloride (AMT) (≥98% TLC powder), and rhodamine B were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cyclohexane (≥99.5%) was from Merk (Darmstadt, Germany), sodium hydroxide was from Riedel-de Haën (Sigma-Aldrich, St. Louis, MO, USA) and methanol (HPLC grade) was from Fisher Chemical (Hampton, VA, USA). All chemicals were used as received. Wherever we mentioned water in this work it means Ultrapure (Type 1) water from Synergy^® UV Water Purification System (Merck, Darmstadt, Germany).

Lipids used in this work are Egg PC l-α-phosphatidylcholine (T_t ≤ 0 °C, EPC), Hydro Soy PC l-α-phosphatidylcholine (T_t = 52 °C, HSPC), cholesterol (Chol), 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (16:0 Liss RhodB-PE, λ_Ex/λ_Em = 560/583 nm) from Avanti Polar Lipids (Alabaster, AL, USA). N-isopropylacrylamide (NIPA) as a monomer, N,N′-methylene-bis-acrylamide (MBA) as a cross linker, fluorescein-o-acrylate (FA, λ_Ex/λ_Em = 490/520 nm) as a fluorescent monomer, ammonium persulfate (APS) as an initiator, N,N,N′,N′-tetramethylenediamine (TEMED) as an accelerator, sorbitane monostearate (Span 60) as a surfactant, 2,2-diethoxyacetophenone (DEAP) as a photoinitiator, and solvents (dimethyl sulfoxide, cyclohexane, chloroform, methanol, and ethanol of chemical grade) are purchased from Sigma Aldrich (Saint Louis, MO, USA). Water purified by RiOs-16 Essential Water Purification System (EMD Millipore, Billerica, MA, USA) at the resistivity of 16 MΩ·cm was used in all experiments. All chemicals are used as purchased without further purification.