GRP Enzyme ImmunoAssay (EIA), that is specific for the mature form of GRP (amide 1–27), was purchased from Phoenix Pharmaceuticals, Inc. (Catalog # EK-027–07; Burlingame, CA). GABA ELISA kit was purchased from Aviva Systems Biology (Catalog # OKEH02564; San Diego, CA). HMGB1 ELISA kit was purchased from IBL International (Catalog # ST51011; Toronto, Ontario, Canada). The 8-hydroxy 2 deoxyguanosine (8-OHdG) ELISA kit was purchased from Abcam (Catalog # ab201734; Branford, CT). GAD ELISA was purchased from Lifeome Biolabs (Catalog # EL009159MO-96; Oceanside, CA). The ELISA kit for IFN-β was purchased from PBL Assay Science (Catalog # 42400; Piscataway, NJ). The ELISA kits for TNF-α (Catalog # MTA00B), IL-1β (Catalog # MLB00C), CCL5/RANTES (Catalog # MMR00), and CCL2/MCP-1 (Catalog # MJE008) were purchased from R&D Systems (Minneapolis, MN). The GRP inhibitor, NSC77427, was made by the Small Molecule Library Reagent Program (National Cancer Institute, Division of Cancer Treatment & Diagnosis/Developmental Therapeutics Program, NIH). Saclofen and baclofen were purchased from Tocris Bioscience (Minneapolis, MN). The TLR4 antibody was purchased from Invitrogen (Catalog # 14–991782; Waltham, MA) and the CD68 antibody purchased from Biolegend (San Diego, CA).
Gaba elisa kit
Manufactured by Aviva Systems Biology
Sourced in United States
The GABA ELISA kit is a quantitative enzyme-linked immunosorbent assay (ELISA) designed to measure the concentration of gamma-aminobutyric acid (GABA) in biological samples. The kit utilizes the competitive ELISA principle to determine the GABA levels in the sample.
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2 protocols using gaba elisa kit
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Immunoassay Panel for Inflammatory Markers
2
Quantifying GABA Levels via ELISA
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GABA detection in both AE and NE was assayed using GABA ELISA Kit (Aviva Systems Biology, San Diego, CA, USA) according to the protocol instructions. Briefly, titrated standards and diluted samples were placed in a microtiter well-plate previously pre-coated with an anti-GABA antibody. GABA-Biotin complex was added to each well and the plate was incubated for 60 min. After incubation, the plate was washed. Avidin-HRP conjugate was added to each well and incubated for 45 min. Finally, 3,3′,5,5′-Tetramethylbenzidine (TMB) substrate was incubated for 30 min. Samples were read at 450 nm on an ELISA standard microplate reader.
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