Rabbit anti human oct4

Proteins were extracted from the cells using RIPA buffer, resolved by SDS-polyacrylamide gels, and then transferred to poly-vinylidene difluoride (PVDF) membranes. The membranes were probed with rabbit anti-human TAZ (1:1000, cat# 83669), rabbit anti-human total caspase-3 (1:1000, cat# 9662), rabbit anti-human cleaved caspase-3 (1:1000, cat# 9661), rabbit anti-human total PARP (1:1000, clone: 46D11, cat# 9532), rabbit anti-human cleaved PARP (1:1000, clone: D64E10, cat# 5625), mouse anti-human NANOG (1:1000, cat# 4893), or rabbit anti-human SOX2 (1:1000, cat# 3579) from Cell Signaling Technology, or rabbit anti-human OCT4 (1:1000, abcam, cat# ab19857), rabbit anti-human NANOG (1:1000, clone: EPR2027(2), cat# ab109250, Abcam), mouse anti-human GAPDH (1:10000, clone: 1E6D9, cat# 60004-1-Ig, Proteintech), or rabbit anti-human α-tubulin (1:10000, cat# 11224-1-AP, Proteintech) antibodies overnight at 4 °C. HRP-linked anti-rabbit IgG (1:3000, cat# 7074, Cell Signaling Technology) or anti-mouse IgG (1:3000, cat# 7076, Cell Signaling Technology) was used and the antigen-antibody reaction was visualized by an enhanced chemiluminescence assay (ECL, Thermo). Densitometry was performed using ImageJ software. GAPDH or α-tubulin run on the same blot were used as the loading controls unless otherwise indicated.