Venous blood samples were obtained from all subjects after at least 12 h of fasting. The levels of serum total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) in samples were determined by enzymatic methods with commercially available kits, Tcho-1, TG-LH (RANDOX Laboratories Ltd., Ardmore, Diamond Road, Crumlin Co., Antrim, UK, BT29 4QY), Cholestest N HDL, and Cholestest LDL (Daiichi Pure Chemicals Co., Ltd., Tokyo, Japan), respectively. Serum apolipoprotein (Apo) A1 and ApoB levels were detected by the immunoturbidimetric immunoassay (RANDOX Laboratories Ltd.). All determinations were performed with an autoanalyzer (Type 7170A; Hitachi Ltd., Tokyo, Japan) in the Clinical Science Experiment Center of the First Affiliated Hospital, Guangxi Medical University [22 (link),23 (link),24 (link),25 (link),26 (link),27 (link),28 (link),29 (link),30 (link),31 (link),32 (link),33 ].
Tcho 1
Manufactured by Randox
Sourced in United Kingdom
Tcho-1 is a laboratory equipment manufactured by Randox. It is designed for the analysis of cholesterol levels in biological samples. The Tcho-1 utilizes a specific analytical method to provide accurate measurements of cholesterol concentrations.
Automatically generated - may contain errors
7 protocols using tcho 1
1
Serum Lipid and Apolipoprotein Profiling
2
Serum Lipid Biomarker Analysis
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Venous blood samples were obtained from all subjects after at least 12 h of fasting. The levels of serum TC, TG, HDL-C, and LDL-C in samples were determined by enzymatic methods with commercially available kits, Tcho-1, TG-LH (Randox Laboratories Ltd., Ardmore, Diamond Road, Crumlin Co., Antrim BT29 4QY, U.K., ), Cholestest N HDL and Cholestest LDL (Daiichi Pure Chemicals Co., Ltd., Tokyo, Japan), respectively. Serum ApoA1 and ApoB levels were detected by the immunoturbidimetric immunoassay (Randox Laboratories Ltd.). All determinations were performed with an autoanalyzer (Type 7170A; Hitachi Ltd., Tokyo, Japan) in the Clinical Science Experiment Center of the First Affiliated Hospital, Guangxi Medical University [33 (link)–36 (link)].
3
Lipid Profiling in Fasted Subjects
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Venous blood samples were collected from all subjects after at least 12 h of fasting. The levels of serum TC, TG, HDL-C, and LDL-C in samples were determined by enzymatic methods with commercially available kits, Tcho-1, TG-LH (RANDOX Laboratories Ltd., Ardmore, Diamond Road, Crumlin Co., Antrim, UK, BT29 4QY), Cholestest N HDL, and Cholestest LDL (Daiichi Pure Chemicals Co., Ltd., Tokyo, Japan), respectively. Serum apolipoprotein (Apo) A1 and ApoB levels were detected by the immunoturbidimetric immunoassay (RANDOX Laboratories Ltd.). All determinations were performed with an autoanalyzer (Type 7170 A; Hitachi Ltd., Tokyo, Japan) in the Clinical Science Experiment Center of the First Affiliated Hospital, Guangxi Medical University46 ,47 (link).
4
Serum Lipid and Apolipoprotein Profiling
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Venous blood samples were acquired following 12 h of fasting. TC, HDL-C, LDL-C and TG concentrations in serum were detected by means of Tcho-1, TG-LH (RANDOX Laboratories, UK), Cholestest N HDL, and Cholestest LDL (Daiichi Pure Chemicals Co., Ltd., Japan) kits, respectively. ApoA1 and ApoB concentrations in serum were determined by immunoassay (RANDOX Laboratories). Detection of all samples was completed with an autoanalyzer (Hitachi Ltd., Japan) [60 (link)].
5
Lipid Panel Measurement Methods
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We received venous blood samples from all subjects after at least 12 h of fasting. The enzymatic methods with commercially available kits, including Tcho-1, TG-LH (RANDOX Laboratories Ltd., Ardmore, Diamond Road, Crumlin Co., Antrim, UK, BT29 4QY), Cholestest N HDL, and Cholestest LDL (Daiichi Pure Chemicals Co., Ltd., Tokyo, Japan) were used for detecting levels of serum TC, TG, HDL-C, and LDL-C, respectively, in samples. However, the immunoturbidimetric immunoassay (RANDOX Laboratories Ltd.) was needed to explore serum ApoA1 and ApoB concentrations. All determinations were performed with an autoanalyzer (Type 7170A; Hitachi Ltd., Tokyo, Japan) in the Clinical Science Experiment Center of the First Affiliated Hospital, Guangxi Medical University [37 (link)–48 ].
6
Serum Lipid and Apolipoprotein Measurements
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Venous blood samples were obtained from all subjects after at least 12 h of fasting. The levels of serum TC, TG, HDL-C, and LDL-C in samples were determined by enzymatic methods with commercially available kits, Tcho-1, TG-LH (RANDOX Laboratories Ltd., Ardmore, Diamond Road, Crumlin Co., Antrim, UK, BT29 4QY), Cholestest N HDL, and Cholestest LDL (Daiichi Pure Chemicals Co., Ltd., Tokyo, Japan), respectively. Serum ApoA1 and ApoB levels were detected by the immunoturbidimetric immunoassay (RANDOX Laboratories Ltd.). All determinations were performed with an autoanalyzer (Type 7170A; Hitachi Ltd., Tokyo, Japan) in the Clinical Science Experiment Center of the First Affiliated Hospital, Guangxi Medical University40 (link).
7
Determining Lipid Biomarkers in Fasting Blood
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Venous blood samples were obtained from all subjects after at least 12 h of fasting. The levels of serum TC, TG, HDL-C and LDL-C in samples were determined by enzymatic methods with commercially available kits, Tcho-1, TG-LH (RANDOX Laboratories Ltd., Ardmore, Diamond Road, Crumlin Co., Antrim, UK, BT29 4QY), Cholestest HDL, and Cholestest LDL (Daiichi Pure Chemicals Co., Ltd., Tokyo, Japan), respectively. Serum ApoA1 and ApoB levels were detected by the immunoturbidimetric immunoassay (RANDOX Laboratories Ltd.). All determinations were performed with an autoanalyzer (Type 7170A; Hitachi Ltd., Tokyo, Japan) in the Clinical Science Experiment Center of the First Affiliated Hospital, Guangxi Medical University [32] .
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