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Anti mhcii e450

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The Anti-MHCII-e450 is a laboratory reagent used to detect and quantify major histocompatibility complex class II (MHCII) molecules. It is a fluorescently labeled antibody that binds to MHCII proteins, allowing for their identification and enumeration in various biological samples.

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Lab products found in correlation

Anti cd11c pecy7, by Thermo Fisher Scientific (1 mentions) Anti cd45 af700, by Thermo Fisher Scientific (1 mentions) Anti gr1 percpcy5, by Thermo Fisher Scientific (1 mentions) Anti cd11b apc, by Thermo Fisher Scientific (1 mentions) Anti ly6c pe, by Thermo Fisher Scientific (1 mentions) Anti cd16 cd32, by BD (1 mentions) Anti cd8 apc, by Thermo Fisher Scientific (1 mentions) Elispot assay, by R&D Systems (1 mentions) Anti cd3 apccy7, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

MHCII (84 citations) Anti-MHCII (34 citations)

2 protocols using anti mhcii e450

1

Multiparametric Flow Cytometry of Murine Lungs

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On day 7, lungs were collected aseptically after mice were euthanized. Lungs were passed through 70 um cell strainer to obtain single-cell homogenates. Red blood cells were lysed and the remaining cells were washed with FACS buffer. After cells were resuspended in 200 ul of FACS buffer, Fc receptors were blocked with anti-CD16/CD32 (Fc Block, BD) and then stained using the following antibodies (all from eBiosciences): anti-CD11b-APC, anti-CD11c-PECy7, anti-CD45-AF700, anti-Gr1-PerCpCy5.5, viability-e520, anti-Ly6C-PE, anti-MHCII-e450, anti-SiglecF-PE-CF594.
Choi A., Ibañez L.I., Strohmeier S., Krammer F., García-Sastre A, & Schotsaert M. (2020). Non-sterilizing, Infection-Permissive Vaccination With Inactivated Influenza Virus Vaccine Reshapes Subsequent Virus Infection-Induced Protective Heterosubtypic Immunity From Cellular to Humoral Cross-Reactive Immune Responses. Frontiers in Immunology, 11, 1166.
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2

ELISPOT Assay for NP-specific CD8+ T Cells

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Mice lung lymphocytes collected for the ELISPOT assay (R&D Systems) were also used to stain NP-specific CD8+ T cells. After lymphocytes were pelleted and resuspended in FACS buffer, Fc receptors were blocked using anti-CD16/CD32 (BD) and were stained using viability stain-e520, anti-MHCII-e450, anti-CD3-APC-Cy7, anti-CD8-APC (all eBiosciences), and NP-specific Pro5 pentamer-PE (NP155-peptide, ProImmune).
Choi A., Ibañez L.I., Strohmeier S., Krammer F., García-Sastre A, & Schotsaert M. (2020). Non-sterilizing, Infection-Permissive Vaccination With Inactivated Influenza Virus Vaccine Reshapes Subsequent Virus Infection-Induced Protective Heterosubtypic Immunity From Cellular to Humoral Cross-Reactive Immune Responses. Frontiers in Immunology, 11, 1166.
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