The fear test was conducted with a fear chamber (Coulbourn Instruments, Holliston, MA, USA) as previously described. Fear conditioning was performed 5-minutes after placing mice in the fear chamber: three trials of a conditioned stimulus (CS, 20-second long, 3 kHz, 80 dB tone) paired with an unconditioned stimulus (US, 1 second long, 0.5 mA electrical shock) at a 60-second interval. Contextual-fear memory was measured 24 hours later after being placed in the same chamber for 5 minutes. Cue-memory was analyzed after another 24 hours by placing the mice in a novel chamber. CS was applied for 3 minutes after a 5-minute habituation period.
Fear chamber
Manufactured by Harvard Apparatus
Sourced in United States
The Fear Chamber is a laboratory instrument designed to study fear-related behaviors in animal models. Its core function is to create a controlled environment where subjects can be exposed to stimuli that elicit fear responses, allowing researchers to observe and analyze these reactions.
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Lab products found in correlation
2 protocols using fear chamber
1
Fear Conditioning and Memory Assessment in Mice
2
Fear Conditioning Protocol in Mice
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Subject mice were placed in the fear chamber (Coulbourn Instruments) and allowed to freely explore for 10 min for habituation. On the next day, mice were allowed to explore the same chamber for 5 min, and five electrical foot shocks signed with tone were given to the subjects (2 s 0.7 mA, 1 min apart) during the last 5 min. After 24 h, mice were returned to the same chamber, and their freezing behaviors were recorded without foot shocks for 5 min. Freezing was defined as the absence of movement longer than 1 s and analyzed automatically using FreezeFrame (Coulbourn Instruments).
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