Ab1598p

Primary antibodies included rabbit anti-M₁ (Abcam, ab111100), rabbit anti-M₅ (Abcam, ab41171), chicken anti-tyrosine hydroxylase (Aves, TYH), rat anti-DAT (Thermo Fisher, mAb16), and rabbit anti-vMAT (Millipore, AB1598P). HRP, Dylight-488, or Alexa 568 conjugated secondary antibodies were from Jackson Immunoresearch. Dynole 34-2 and Pitstop 2 and the negative control were both from Abcam. Custom-designed TAT-peptides were ordered from LifeTein.

Unless otherwise specified, all chemicals and reagents were purchased from Sigma-Aldrich. Rabbit anti-tyrosine hydroxylase (TH) primary antibody was purchased from Millipore (MAB152, AB_390204) and used at a 1:2000 dilution. Rabbit anti-Ser40 p-TH was purchased from Cell Signaling (#2791, AB_2201522) and used at a 1:1000 dilution. Rat anti-DAT was purchased from Millipore (MAB369, AB_2190413) and used at a 1:1000 dilution. Rabbit anti-VMAT2 was purchased from Millipore (AB1598P, AB_2285927) and used at a dilution of 1:1000. Rabbit anti-COMT was purchased from Abcam (ab126618, AB_11129919) and used at a dilution of 1:4000. Mouse anti-GAPDH was purchased from Sigma Aldrich (G8795, AB_1078991) and used at a dilution of 1:4000. All primary antibodies were diluted in 5% BSA in TBS-T. Secondary antibodies linked to horse radish peroxidase were purchased from Cell Signaling (rabbit: #7074s; mouse: #7076; rat: #7077) and diluted to 1:4000 before use in 5% nonfat milk in TBS-T.