Wizard magnetic kit

Young leaves were collected from parental and F₂ lines. Plant genomic DNA was isolated using the Wizard Magnetic Kit (Promega, Madison, WI, United States) following the instructions of manufacturer. DNA was extracted from each individual plant lines, and a gel electrophoresis was performed to determine whether high molecular weight DNAs were isolated. The resistant and susceptible bulks were generated from 20 resistant and 20 susceptible F₂ individuals, respectively, as described in earlier studies (Devran et al., 2015 (link), 2018 (link)). Genomic DNA library and sequencing have been carried out by the University of Exeter Sequencing Service after quality check of DNAs, generating 2 ×150 bp paired-end read data for each parent line and bulked (resistant and susceptible) pools with Illumina HiSeq 2500 (Illumina, Inc. San Diego, CA, USA).

Young leaves were collected from plants after pathotyping with FORL. Plant genomic DNA was then isolated using the Wizard Magnetic Kit (Promega) following the manufacturer’s instructions. DNA was extracted separately from each individual of the F₂ plant lines. The resistant and susceptible bulks were generated from twenty resistant and twenty susceptible F₂ individuals, respectively, as described (Devran et al. 2015 (link)) and used for genomic sequencing. We generated 1 lane of 100 bp paired-end Illumina HiSeq 2500 sequencing data for each parent (resistant and susceptible) line and bulked (resistant and susceptible) pools.

Genomic DNA was extracted from young leaves collected from parental lines and F₂ plants using the Wizard Magnetic Kit (Promega) following the manufacturer’s instructions. DNA was then run on an agarose gel and checked for degradation or their high molecular weight. The quality control (QC) of the DNA was determined by Novogene UK (Cambridge, United Kingdom) before proceeding to the sequencing.