All experiments were approved by the Institutional Animal Care and Use Committee of Ajou University (IACUC No. 2014-0038). WT C57BL/6 mice were purchased from Charles River Laboratories (Orient Bio Inc., Sungnam, Korea). CX3CR1(GFP/+) mice, MyD88 knockout (KO) mice, CD45.1+ C57BL/6 mice and CCR7 KO mice (C57BL/6 background) were purchased from Jackson Laboratories (Bar Harbor, ME). CX3CR1-DTR mice (C57BL/6 background) were kindly provided by Dan R. Littman (New York University Medical Center) and Charles Surh (Pohang University of Science and Technology) (20 (link)). All mice used in the study were at 6 weeks of age. The mice were kept in the Laboratory Animal Research Center of Ajou University Medical Center.
Myd88 knockout ko mice
Manufactured by Jackson ImmunoResearch
Sourced in United States
MyD88 knockout (KO) mice are a laboratory animal model where the MyD88 gene has been genetically modified to be non-functional. The MyD88 protein plays a central role in the innate immune system's response to pathogens. These mice can be used to study the role of the MyD88 signaling pathway in various biological processes.
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Lab products found in correlation
C57bl 6 cd45.1 mice, by Jackson ImmunoResearch (1 mentions)
C57bl 6 wt mice, by Charles River Laboratories (1 mentions)
Cx3cr1 gfp mice, by Jackson ImmunoResearch (1 mentions)
Balb c, by Charles River Laboratories (1 mentions)
C57bl 6 mice, by Charles River Laboratories (1 mentions)
C57bl 6 mice, by Jackson ImmunoResearch (1 mentions)
4 protocols using myd88 knockout ko mice
1
Mouse Immune Cell Trafficking Protocol
2
Myd88 Knockout Mice Behavioral Study
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We used adult male Myd88 knockout (KO) mice (provided by Dr. Blednov from the University of Texas at Austin, Austin, TX; originated from Jackson Laboratories; n = 19; age: 28.7 ± 1.4 weeks old; weight: 30.53 ± 0.65 g) and C57BL/6J wildtype (WT) control male mice (Jackson Laboratories; n = 17; age: 25.8 ± 1.6 weeks old; weight: 31.47 ± 0.97 g). The mice were housed in a temperature- and humidity-controlled room on a 12 h light cycle (lights on from 06:00–18:00), and food and water were available ad libitum. In our care of the mice, we followed the National Institutes of Health Guide for the Care and Use of Laboratory Animals and the Institutional Animal Care and Use Committee policies of The Scripps Research Institute (animal protocol #09-0006-4 pproved on 28 September 2017).
3
Intradermal Immunization of Mice
4
Influenza Viral Challenge in Mice
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C57BL/6 mice (male, 6–8 weeks old) were purchased from Jackson Laboratory (Bar Harbor, ME, USA). MyD88 knockout (KO) mice were purchased from Jackson Laboratory (Bar Harbor, ME, USA) and self-bred for use in this study. Anesthesia was induced by intraperitoneal injection of a mixture of ketamine (80 mg/kg) and xylazine (10 mg/kg). Influenza viral challenge studies were performed in the animal biosafety level 2 (ABSL-2) facility of the University of Rhode Island (URI). All animal procedures were approved by the Institutional Animal Care and Use Committee (IACUC) of URI (AN1415-009) and conducted in accordance with national and institutional guidelines and regulations.
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