Hypersil gold hplc column

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Hypersil GOLD™ HPLC column is a reversed-phase high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a wide range of organic compounds. It features a spherical silica-based stationary phase with a bonded alkyl ligand, providing high efficiency and reproducible separations.

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Lab products found in correlation

Ultimate 3000, by Thermo Fisher Scientific (1 mentions) Orbitrap q exactive series mass spectrometer, by Thermo Fisher Scientific (1 mentions) Vanquish uhplc system, by Thermo Fisher Scientific (1 mentions) Qtrap 6500, by AB Sciex (1 mentions) Tsq vantage triple, by Thermo Fisher Scientific (1 mentions) Accela uhplc system, by Thermo Fisher Scientific (1 mentions) Lcquan software, by Thermo Fisher Scientific (1 mentions) Prism v5.0d, by GraphPad (1 mentions) Qstar elite, by AB Sciex (1 mentions)

Spelling variants of this product

Hypersil GOLD column (97 citations) Hypersil Gold (88 citations) Hypersil Gold reversed phase HPLC C18 column (4 citations) Hypersil GOLD™ C18 HPLC column (3 citations) Hypersil GOLD™ C18 reverse-phase HPLC column (2 citations)

6 protocols using hypersil gold hplc column

Quantitative Analysis of Amoxicillin Compounds

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The high pressure liquid chromatographic U-HPLC-DAD RS system model used (Thermo Ultimate 3000) is equipped with quaternary pump, auto sampler, and thermo stated column compartment with a variable wavelength detector controlled by the Chem station software. The HPLC system has a photodiode array detector (DAD) used for quantification studies. A C18 Thermo Scientific™ Hypersil GOLD™ HPLC column, small and meant for high pressure (100 × 2.1 mm), packed with particles of silica, spherical fully porous ultrapure (1.9 μm) was used as a stationary phase. For amoxicillin trihydrate and amoxicillin trihydrate-clavulanate potassium, the analysis was carried out on binary system using the C18 column (250 × 4.0 mm, 4 μm).

Hobeika E., Farhat J., Saab J., Hleihel W., Azzi-Achkouty S., Sili G., Hallit S, & Salameh P. (2020). Are antibiotics substandard in Lebanon? Quantification of active pharmaceutical ingredients between brand and generics of selected antibiotics. BMC Pharmacology & Toxicology, 21, 15.

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UHPLC-MS/MS Analysis of Metabolites

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Liquid chromatography–tandem mass spectrometry analyses were performed using the Vanquish UHPLC system (Thermo Fisher Scientific, Shanghai, China) coupled with the Orbitrap Q Exactive series mass spectrometer (Thermo Fisher Scientific, Shanghai, China). A Hypersil Gold HPLC column (Thermo Fisher Scientific; 100 mm × 2.1 mm, 1.9 μm) with a 16-min linear gradient and 0.2 ml/min flow rate was employed. Eluent A (0.1% formic acid in water) and eluent B (methanol) were used for positive polarity mode. Eluent A (5 mM ammonium acetate, pH 9.0) and eluent B (methanol) were used for negative polarity mode. The following solvent gradient was used: 2% eluent B, 1.5 min; 2–100% eluent B, 12.0 min; 100% eluent B, 14.0 min; 100–2% eluent B, 14.1 min; and 2% eluent B, 17 min. The mass spectrometer was operated in positive/negative polarity mode with the following settings: spray voltage = 3.2 kV, capillary temperature = 320°C, sheath gas flow rate = 35 arbitrary unit (arb), and auxiliary gas flow rate = 10 arb.

Li S., Du M., Zhang C., Wang Y., Lee Y, & Zhang G. (2022). Diet Type Impacts Production Performance of Fattening Lambs by Manipulating the Ruminal Microbiota and Metabolome. Frontiers in Microbiology, 13, 824001.

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Metabolomic Analysis of ARDS Samples

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Blood samples and bronchoalveolar lavage fluid (BALF) were collected within 9 h of ARDS diagnosis for metabolomic analysis. Collectively, 59 patients were enrolled in this study. Blood samples(2 mL) were collected in heparin tubes and centrifuged at 500×g at 4°C for 5 min, the supernatants were extracted and stored at −80°C untill further use. Phenylalanine levels were assessed using mass spectrometry/high-performance liquid chromatography (Hypersil GOLD HPLC column [ThermoFisher, Waltham, MA, USA] coupled to a QTRAP 6500 [SCIEX, Framingham, MA, USA]). by Mass Spectrometry Platform, National Research Center for Translational Medicine, Ruijin Hospital Affiliated to Shanghai Jiao Tong University (SJTU) School of Medicine, Shanghai, China.

Tang Y., Yu Y., Li R., Tao Z., Zhang L., Wang X., Qi X., Li Y., Meng T., Qu H., Zhou M., Xu J, & Liu J. (2023). Phenylalanine promotes alveolar macrophage pyroptosis via the activation of CaSR in ARDS. Frontiers in Immunology, 14, 1114129.

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Quantitative LC-MS/MS Analysis of Tofacitinib

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LC-MS/MS analysis was performed on a TSQ Vantage triple quadrupole mass spectrometer coupled with an Accela UHPLC system (Thermo Fisher Scientific, Massachusetts, USA). Chromatographic separation was performed using a Thermo Scientific™ Hypersil GOLD™ HPLC column with a particle size 1.9 μm, 100 mm length and 2.1 mm diameter maintained at 25 °C. 10 μl of sample was injected onto the column. The mobile phase consisted of ACN with 0.1% formic acid as the organic component (B) and water with 0.1% formic acid as the aqueous phase (A). The system was maintained in 8% buffer B at a flow rate of 0.3 ml min⁻¹. Samples were maintained at 4 °C prior to analysis.
Tofacitinib and tofacitinib-d3 were detected using heated electrospray ionisation in positive ion mode using the following selected reaction monitoring (SRM) transitions: 313.0 > 173.06 for tofacitinib and 316.3 > 176.1 for tofacitinib-d3. The mass spectrometer settings were optimised as follows: spray voltage 5000 V, capillary temperature 369 °C, vaporiser temperature 353 °C and collision energy 29 eV. Argon was used as collision gas. Quantitation was calculated using the peak-area ratio of the analyte to internal standard using LCQuan software (Thermo Fisher Scientific, MA, USA).

Church S., Hyrich K.L., Ogungbenro K., Unwin R.D., Barton A, & Bluett J. (2023). Development of a sensitive biochemical assay for the detection of tofacitinib adherence. Analytical Methods, 15(14), 1797-1801.

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Zebrafish Cyp27c1-Catalyzed Retinoid Oxidation

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The reaction mixtures contained recombinant zebrafish Cyp27c1, bovine adrenodoxin (Adx), bovine NADPH-adrenodoxin reductase (ADR), potassium phosphate (50 mM), and various concentrations of a retinoid substrate, which were added to an amber vial and pre-incubated in a water bath at 37°C prior to initiation with an NADPH generating system. Following a 60 second incubation, the reaction was quenched with 1.0 mL of ethyl acetate solution containing 45 μM butylated hydroxytoluene (to prevent radical reactions). The organic solvent was extracted, dried under nitrogen and resuspended in CH₃CN (100 μL). HPLC-UV was used to separate and detect the products. Briefly, 10 μL of the sample was injected onto a Hypersil GOLD HPLC column (150 mm × 2.1 mm, 3 μm, Thermo Scientific) at 40°C and the products were eluted isocratically (0.5 mL/min) at 30% solvent A (95% H₂O; 4.9% CH₃CN; 0.1% formic acid, v/v) and 70% solvent B (95% CH₃CN; 4.9% H₂O; 0.1% formic acid, v/v) at a flow rate of 0.5 mL/min. Data analysis and graph generation was conducted using GraphPad Prism v. 5.0d, using a single site hyperbolic equation.

Enright J.M., Toomey M.B., Sato S.Y., Temple S.E., Allen J.R., Fujiwara R., Kramlinger V.M., Nagy L.D., Johnson K.M., Xiao Y., How M.J., Johnson S.L., Roberts N.W., Kefalov V.J., Guengerich F.P, & Corbo J.C. (2015). Cyp27c1 red-shifts the spectral sensitivity of photoreceptors by converting vitamin A1 into A2. Current biology : CB, 25(23), 3048-3057.

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HPLC-MS Optimization for Non-Derivatized Peptides

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A 10 cm, 1.0 mm I.D. Hypersil Gold HPLC column (Thermo Fisher Scientific, Inc., USA) with C18, 175 Å, and 3 μm resin was used. Solvent A was composed of 98.8% H₂O, 1.0% ACN, and 0.2% FA and solvent B was 98.8% ACN, 1.0% H₂O, and 0.2% FA. The LC gradient was 5% to 35% B for 45 minutes. The column temperature was 60 °C. The hybrid mass spectrometer used was a QSTAR^® Elite (AB Sciex, Framingham, MA). Positive electrospray ionization (ESI) was used, with parameters of 5.5 kV for the spray voltage and source temperature of 300 °C. The initial collision energy (CE) and declustering potential (DP) were obtained for the doubly- and triply-charged peptides from Equations 1 and 2, respectively [25 (link)].
These equations were empirically adjusted for the non-derivatized peptides (Table S1), and for dim-2-peptides (Table S2). The new equations from the dim-2-peptides were applied to dim-3-through dim-6-peptides.

McShane A.J., Shen Y., Castillo M.J, & Yao X. (2014). Peptide Dimethylation: Fragmentation Control via Distancing the Dimethylamino Group. Journal of the American Society for Mass Spectrometry, 25(10), 1694-1704.

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