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Respective secondary antibodies conjugated flours

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Respective secondary antibodies conjugated with fluorescent dyes. These products are designed to detect and visualize primary antibodies in various immunological applications.

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Lab products found in correlation

Muc5ac, by Merck Group (2 mentions) β tubulin, by Cell Signaling Technology (2 mentions) Bzx700 microscopy system, by Keyence (2 mentions)

2 protocols using respective secondary antibodies conjugated flours

1

Immunohistochemical Analysis of Airway Tissues

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For immunohistochemical staining, deparaffinized and hydrated airway tissue sections and treated HAECs were washed in 0.05% v Brij-35 in PBS (pH 7.4) and immunostained as described previously.50 (link) Briefly, antigens were unmasked by steaming sections in 10 mM Citrate buffer (pH 6.0) followed by incubation in a permeabilizing blocking solution, and sections were stained with antibodies to MUC5AC (Millipore Inc., MA), and β-tubulin (Cell Signaling Tech, MA) or isotype controls. The immunolabelled cells were detected using respective secondary antibodies conjugated flours (Jackson ImmunoResearch Lab Inc., West Grove, PA) and mounted with 4’,6-diamidino-2-phenylindole (DAPI) containing Fluormount-G™ (SouthemBiotech, Birmingham, AL) for nuclear staining. Immunofluorescent images were captured using BZX700 Microscopy system (Keyence) and analyzed using NIH Image J software.
Devadoss D., Daly G., Manevski M., Houserova D., Hussain S.S., Baumlin N., Salathe M., Borchert G., Langley R.J, & Chand H.S. (2020). A Long Noncoding RNA Antisense to ICAM-1 is Involved in Allergic Asthma Associated Hyperreactive Response of Airway Epithelial Cells. Mucosal immunology, 14(3), 630-639.
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2

Immunohistochemical Analysis of Airway Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
For immunohistochemical staining, deparaffinized and hydrated airway tissue sections and treated HAECs were washed in 0.05% v Brij-35 in PBS (pH 7.4) and immunostained as described previously.50 (link) Briefly, antigens were unmasked by steaming sections in 10 mM Citrate buffer (pH 6.0) followed by incubation in a permeabilizing blocking solution, and sections were stained with antibodies to MUC5AC (Millipore Inc., MA), and β-tubulin (Cell Signaling Tech, MA) or isotype controls. The immunolabelled cells were detected using respective secondary antibodies conjugated flours (Jackson ImmunoResearch Lab Inc., West Grove, PA) and mounted with 4’,6-diamidino-2-phenylindole (DAPI) containing Fluormount-G™ (SouthemBiotech, Birmingham, AL) for nuclear staining. Immunofluorescent images were captured using BZX700 Microscopy system (Keyence) and analyzed using NIH Image J software.
Devadoss D., Daly G., Manevski M., Houserova D., Hussain S.S., Baumlin N., Salathe M., Borchert G., Langley R.J, & Chand H.S. (2020). A Long Noncoding RNA Antisense to ICAM-1 is Involved in Allergic Asthma Associated Hyperreactive Response of Airway Epithelial Cells. Mucosal immunology, 14(3), 630-639.
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