Desugared egg white protein (EWP) powders were obtained from Rongda Poultry Co., Ltd. (Anhui province, China). Catechin (CT, purity ≥ 98%) and epigallocatechin gallate (EGCG, purity ≥ 98%) were purchased from Macklin Co., Ltd. (Shanghai, China). 2-Diphenyl-1-picrylhydrazyl (DPPH), 8-anilino-1-naphthalenesulfonic acid (ANS), Coomassie Brilliant Blue R-250 and dialysis bags (MWCO 10000 Da) were purchased from Sigma Chemical Co. (St. Louis, USA). All the other chemical reagents were of analytical grade and purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
Epigallocatechin gallate (egcg)
Manufactured by Maclin Biochemical Technology
Sourced in China
EGCG is a biochemical compound that can be used in various laboratory applications. It functions as a polyphenol with antioxidant properties. The core purpose of EGCG is to serve as a research tool for scientific investigations.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions)
Pen strep, by Thermo Fisher Scientific (2 mentions)
Calcein pi live dead viability assay kit, by Beyotime (1 mentions)
Calcium colorimetric assay kit, by Beyotime (1 mentions)
Cck8 reagent, by Fdbio (1 mentions)
Crystal violet, by Beyotime (1 mentions)
Sodium dodecyl sulfate (sds), by Maclin Biochemical Technology (1 mentions)
Dnase, by Yuanye Bio-Technology (1 mentions)
Triton x 100, by Shanghai Titan Scientific (1 mentions)
Fetal bovine serum (fbs), by Beyotime (1 mentions)
Anti caspase 3, by Merck Group (1 mentions)
Anti gfap, by Merck Group (1 mentions)
Anti iba1, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Anti neun, by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole dihydrochloride dapi, by Solarbio (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Solarbio (1 mentions)
Phosphate buffer saline (pbs), by Wuhan Servicebio Technology (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Maclin Biochemical Technology (1 mentions)
12 protocols using epigallocatechin gallate (egcg)
1
Desugared Egg White Protein Powders: Antioxidant Properties
2
Cytocompatible Polymer-Based Nanocomposite
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PCLA was synthesized by ring‐opening the bulk polymerization of caprolactone (CL) and lactide (LA), provided by Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences (Viscosity: 2.2‐1.4). The acicular nano‐hydroxyapatite (HA), and Epigallocatechin gallate (EGCG) were purchased from Shanghai Macklin Biochemical Co., Ltd. Acetic acid and polyvinyl alcohol (PVA, 101–110 KDa, 87%–89% hydrolyzed) were obtained from Maya Reagent Co., China. Silane Coupling Agent (KH550) was purchased from Beijing bainuowei biology science and technology Co., ltd. CCK‐8 reagent (Fdbio science), Calcein/PI Live/Dead Viability Assay Kit, crystal violet and Calcium Colorimetric Assay Kit were purchased from Beyotime Biotechnology (China). MC3T3‐E1, HepG2 and A549 cell lines were acquired from ATCC. Cell culture medium and fetal bovine serum (FBS) were supplied from HyClone (USA). Ultrapure water (18 MΩ) was used throughout the study.
3
Silver Carp Biomolecule Extraction
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FSBs of silver carp were purchased from a local aquatic product market (Shanghai, China). Sodium dodecyl sulfate (SDS) was purchased from Shanghai Macklin Biochemical Co. (Shanghai, China). Triton X-100 was obtained from Shanghai Titan Scientific Co. (Shanghai, China). DNase and RNase were purchased from Shanghai Yuanye Bio-Technology Co. (Shanghai, China). EGCG, PC, PCA and TA were obtained from Shanghai Macklin Biochemical Co. (Shanghai, China). The cell proliferation and cytotoxicity assay kit (CCK-8 kit) and fetal bovine serum (FBS) were obtained from Beyotime Biotech (Shanghai, China). The aforementioned chemicals were used as received without further purification.
4
Antioxidant and Neuroprotective Effects
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EGCG and selenious acid were obtained from Shanghai Macklin Biochemical (Shanghai, China), 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) were obtained from Solarbio Science & Technology (Beijing, China), sodium ascorbate was obtained from Aladdin Bio-Chem Technology (Shanghai, China), and Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum were obtained from Thermo Fisher Scientific (Waltham, MA). Anti-Iba-1, anti-caspase 3, anti-NeuN, anti-GFAP, and anti-GPX 1 antibody were bought from Sigma-Aldrich (St. Louis, MO). Anti-CD68, anti-NF200, and anti-superoxide dismutase (SOD) antibodies were bought from Abcam (Cambridge, United Kingdom). Hydrogen peroxide (H2O2, 30 wt% in water) was bought from Shanghai Macklin Biochemical.
5
Synthesis and Characterization of BLM Nanoparticles
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BLM was purchased from Acmec biochemical (China). Bovine serum albumin (BSA) and EGCG were purchased from Macklin (China). FeCl3·6H2O was purchased from Aladdin (China). Phosphate buffer saline (PBS) was purchased from Servicebio (China). Penicillin–streptomycin solution (100 ×), fetal bovine serum (FBS), RPMI medium 1640, and trypsin were purchased from Gibco (USA). 4′,6-diamidino-2-phenylindole (DAPI), fluorescent dye 1,1′-dioctadecyl-3,3,3′-dio tetramethylindotricarbocyanine iodide (DiR) and ROS assay kit were obtained from Beyotime (China). Ethanol absolute, ammonia solution, ethylenediamine tetraacetic acid (EDTA), urea, glucose and all other chemical reagents were purchased from Sinopharm Chemical Reagent Co., Ltd (China).
6
Biomolecular Extraction and Characterization
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β-LG from bovine milk (purity ≥ 95%), EGCG (purity ≥ 98%), and CA (purity ≥ 98%) were purchased from Shanghai Macklin Biochemical Co., Ltd (Shanghai, China). 8-anilion-1-naphhalenesulfonic acid ammonium salt (ANS) was a product from Aladdin Industrial Corporation (Shanghai, China). Soybean oil was obtained from Cofco Donghai Grain and Oil Industry (Zhangjiagang) Co., Ltd. (Jiangsu, China). Ultrapure (UP) water purified from a Millipore Milli-Q Advantage A10 Water Purification System (Bedford, MA, USA) was used throughout. All other chemicals and reagents were of analytical reagents and were bought from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China) unless otherwise specified.
7
Hyaluronic Acid and Konjac Gum Composite Synthesis
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HA (molecular weight: 200–400 kDa), sodium periodate, EGCG, and hydroxylamine hydrochloride were purchased from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China). KGM (molecular weight: 200–2000 kDa) was purchased from Zhaotong Sanai Organic Konjac Development Co., Ltd. (Yunnan, China). Glycol and anhydrous sodium carbonate were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). The dialysis bag (MW 3.5 kDa cut-off) was purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China).
8
Antioxidant and Anti-inflammatory Effects
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Active baker's Yeast was purchased from Angel Yeast Co., Ltd. (Yichang, China). Curcumin and EGCG were purchased from Macklin (Shanghai, China). DSS was purchased from Yeasen (Shanghai, China). Phosphate buffer saline (PBS) was purchased from Sevier (Wuhan, China). 2,7-dichlorofluorescein diacetate (DCFH-DA) was purchased from Beyotime Biotechnology Co., Ltd. Fluorescent lipophilic dyes (DiL and DiR) were purchased from Promokine (Heidelberg, Germany). MTT, myeloperoxidase (MPO) assay kit, lipopolysaccharides (LPS), and antibodies (CD86, CD80, and CD206) were purchased from Meilun Bio (Dalian, China).
9
Isolation and Characterization of Nemopilema nomurai Tentacles
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EGCG (purity 95%) was purchased from Shanghai Macklin Biochemical Co., Ltd. Percoll (1.129 g/mL) was purchased from GE Healthcare Life Sciences. Phosphate buffered saline (PBS) was purchased from Shanghai Beyotime Biotechnology Co., Ltd. (Shanghai, China). Heparin sodium (150 u/mg) was purchased from Shanghai Yuanye Biotechnology Co., Ltd. Cell Counting Kit-8 was purchased from Dojindo China Co., Ltd. (Shanghai, China). Fetal bovine serum (FBS) and pen strep were obtained from Thermo Fisher Scientific Co., Ltd. (Waltham, MA, USA).
Specimens of the jellyfish Nemopilema nomurai were collected from Laoshan Bay in Qingdao, China, in August 2020. The jellyfish tentacles were excised immediately with scissors from living specimens. The isolated tentacles were placed in plastic bags with dry ice and immediately transported back to the laboratory, where the samples were stored in a −80 ℃ freezer until use.
Specimens of the jellyfish Nemopilema nomurai were collected from Laoshan Bay in Qingdao, China, in August 2020. The jellyfish tentacles were excised immediately with scissors from living specimens. The isolated tentacles were placed in plastic bags with dry ice and immediately transported back to the laboratory, where the samples were stored in a −80 ℃ freezer until use.
10
Bioactive Hydrogel Synthesis and Characterization
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2-Hydroxylethyl methacrylate (HEMA), Ipomoea batatas starch and EGCG were obtained from Macklin (Shanghai, China). Methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA) were obtained from ThermoFisher (United States). 2,2′-azobis (2-methylpropionitrile) (AIBN) was obtained from Sigma-Aldrich (United States) and deionized water was used in the study.
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