Lumina 3 xr

2.5×10⁵ U266 cells were transplanted into NSG mice by tail vein injection. Mice were imaged using a Lumina III XR bioluminescent imaging system (Perkin Elmer, Waltham, MA, USA) starting at 4 weeks post-transplant. Briefly, mice were injected intraperitoneally with sufficient luciferin (Perkin Elmer) for a final concentration of 30 mg/kg. After 10 min, mice were anaesthetized by inhalation of isofluorane and placed in the instrument. To acquire and analyse bioluminescent data, Living Image software (Perkin Elmer) was used. Regions of interest were drawn to encompass the entire mouse and total flux data were recorded.

The method of making PDXv2 mouse models has been previously described^{9 (link)}. Briefly, patient-derived tumor sample (M151002) was transduced with a pHAGE-luc-GFP virus and transplanted subcutaneously on the flanks of the immune deficient mice. Once tumor growth was confirmed by bioluminescence measurements (PerkinElmer IVIS Lumina III XR), the mice were separated in four treatment groups of four mice receiving either karonudib or vehicle (same concentrations and dosing schedule as in the pre-clinical PDX trial) with or without TILs (one injection of 20*10⁶ TILs). Tumor growth was followed by caliper and bioluminescence measurements.