Cisplatin (cis-dichlorodiammine platinum(II), cDDP, >99.9%), minimum essential medium (MEM), non-essential amino acids (NEAA), oxaliplatin (OXA), penicillin/streptomycin 100× solution, phosphate buffered saline (PBS), potassium tetrachloroplatinate(II) (K2PtCl4, >99.9%), potassium tetrachloropalladate(II) (K2PdCl4, >99.9%), N,N′-bis(3-aminopropyl)-1,4-diaminobutane (spermine, free base, Spm), sodium pyruvate (100 mM), trypan blue (0.4% w/v), trypsin-EDTA (1×), as well as solvents, inorganic salts and acids (all of analytical grade) were purchased from Sigma-Aldrich Chemical S.A. (Sintra, Portugal). Fetal bovine serum (FBS) was obtained from Gibco-Life Technologies (Porto, Portugal). Pt2Spm and Pd2Spm were synthesized according to published procedures [48 ] optimized by the authors [16 (link)]. Briefly, 2 mmol of K2PdCl4 or K2PtCl4 were dissolved in a small amount of water, and 1 mmol Spm aqueous solution was added dropwise under stirring. After 24 h, the resulting powder was filtered and washed with acetone. For drug administration, initial stock solutions of cDDP (960 µM), OXA (1 mM), Pt2Spm (2.4 mM) and Pd2Spm (960 µM) were prepared in PBS, for the first two, and PBS/DMSO (20%), for the last two. All solutions were filtered (0.22 µm filter) and stored at 4 °C.
N n bis 3 aminopropyl 1 4 diaminobutane
Manufactured by Merck Group
Sourced in Portugal
N,N′-bis(3-aminopropyl)-1,4-diaminobutane is a chemical compound with the formula C12H32N4. It is a clear, viscous liquid at room temperature. The compound is used as a building block in the synthesis of various chemical products.
Automatically generated - may contain errors
Lab products found in correlation
Potassium tetrachloropalladate, by Merck Group (2 mentions)
Trypan blue, by Merck Group (2 mentions)
Penicillin streptomycin 100 solution, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Trypsin edta, by Merck Group (2 mentions)
Cisplatin, by Merck Group (2 mentions)
Phosphate buffered saline (pbs), by Merck Group (2 mentions)
Sodium pyruvate, by Merck Group (1 mentions)
Potassium tetrachloroplatinate 2, by Merck Group (1 mentions)
Dimethyl sulphoxide, by Merck Group (1 mentions)
Rpmi 1640 medium, by Merck Group (1 mentions)
2 protocols using n n bis 3 aminopropyl 1 4 diaminobutane
1
Platinum and Palladium Complexes Evaluation
2
Cytotoxicity of Cisplatin and Pd2Spm
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Cisplatin (cis-dichlorodiammine platinum(II), >99.9%), (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethyl-sulphoxide (DMSO), penicillin/streptomycin 100× solution, phosphate buffered saline (PBS), potassium tetrachloropalladate(II) (K2PdCl4, >99.9%), RPMI-1640 medium, N,N´-bis(3-aminopropyl)-1,4-diaminobutane (spermine, free base, Spm), Trypan blue (0.4% w/v), trypsin-EDTA (1×), as well as solvents, inorganic salts and acids, were purchased from Sigma-Aldrich Chemical S.A. (Sintra, Portugal). Fetal bovine serum (FBS) was obtained from Gibco-Life Technologies (Porto, Portugal). All reagents were of analytical grade. Pd2Spm was synthesized according to published procedures [45 ] optimized by the authors [46 (link)].
For drug administration, initial stock solutions of Cisplatin (320 µM) were prepared in PBS and Pd2Spm (320 µM) was prepared in PBS with a 10% (v/v) DMSO. All solutions were sterile filtered and stored at −20 °C.
For drug administration, initial stock solutions of Cisplatin (320 µM) were prepared in PBS and Pd2Spm (320 µM) was prepared in PBS with a 10% (v/v) DMSO. All solutions were sterile filtered and stored at −20 °C.
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