Lipopolysaccharide (LPS), N-(1-naphthyl) ethylenediamine, sodium nitrite, and sulfanilamide were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). All other chemicals and reagents used in the study were of analytical grade or higher.
N 1 naphthyl ethylenediamine
Manufactured by Fujifilm
Sourced in Japan
N-(1-naphthyl) ethylenediamine is a chemical compound used as a reagent in laboratory settings. It serves as a detection and quantification agent for various analytes. The core function of this product is to facilitate analytical procedures, but its specific applications and intended uses should not be extrapolated beyond this factual description.
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2 protocols using n 1 naphthyl ethylenediamine
1
Lipopolysaccharide Quantification Protocol
2
Nitric Oxide Quantification in Cell Culture
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After incubation of MG6 cells for 24 h, NO concentration in the medium was measured by the Griess reaction method [28 (link)]. In brief, 100 μL of culture medium was applied to each well of a 96-well plate. Then, 100 μL of an equal mixture of Griess reagent:0.2% N-1-naphthyl ethylenediamine (Wako, Osaka, Japan) and 2% sulfanilamide (Sigma-Aldrich) in 10% H3PO4 (Wako) was added to each well. After incubation for 10 min at room temperature, the absorbance was measured at 550 nm using the microplate reader.
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