Irdye conjugates 800cw and 680cw

Commercial antibodies were purchased from following companies. Cell Signaling: COX IV (3E11), HSP60 (D307), GAPDH (1410C), LC3A/B (#4108), PARP (#9542), Ubiquitin (P4D1); nanoTools: LC3 (#0321-100); Proteintech: PPAN (#11006-1-AP); BD: TIM23 (#611222); Santa Cruz: LAMP2 (H4B3), TOM20 (FL-145); Sigma: GST (G1160, clone2); Abcam: p62 (EPR4844). Secondary antibodies were IRDye conjugates 800CW and 680CW (Li-COR) for Western blotting or rabbit Alexa 488 (Life Technologies), Alexa 488, Alexa 594, Cy2 and Cy3 conjugates (Dianova) for immunofluorescence staining. DAPI mounting medium was purchased from Dianova. Proteinase K (PK) was from NEB, BafA1 (cat. no. 54645) from Cell Signaling, Oligomycin (cat. no. 1404-19-9) was from Calbiochem, Antimycin A (cat. no. ALX-380-075-M010) and staurosporine (cat. no. ALX-380-014-L250) were from Enzo Life Sciences, CQ (cat. no. C66289) and CCCP (cat. no. C2759) were from Sigma.

Commercial antibodies were obtained from following companies: Abnova (Taipei City, Taiwan): UBTF (H00007343-M01), Cell Signaling (Danvers, MA, USA): GAPDH (1410C), p21 (2946); Proteintech (Rosemont, CA, USA): PPAN (11006-1-AP), ATG7 (67341-1-Ig) and SBDS (67200-1-Ig); Sigma (Steinheim, Germany): PES1 (SAB1400457); Thermo Fisher Scientific (Dreieich, Germany): NPM (FC-61991); Roche (Mannheim, Germany): GFP (11814460001); Santa Cruz (Dallas, TX, USA): p53 (sc-126). Secondary antibodies for Western blotting were IRDye conjugates 800CW and 680CW from Li-COR (Lincoln, NE, USA) or Alexa Fluor 594 (Dianova, Hamburg, Germany) for immunofluorescence experiments. DAPI mounting medium were purchased from Dianova (Hamburg, Germany).