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Anti bcma

Manufactured by BioLegend
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Anti-BCMA is a monoclonal antibody that binds to the B-cell maturation antigen (BCMA). BCMA is a cell surface receptor that is expressed on mature B cells and plasma cells. The function of this product is to detect and bind to BCMA.

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Lab products found in correlation

Coulter fc500 flow cytometer, by Beckman Coulter (1 mentions)

Spelling variants of this product

Anti-BCMA antibody (2 citations) Anti-BCMA antibody conjugated to PE (2 citations) Anti-BCMA-PE (2 citations)

2 protocols using anti bcma

1

Flow Cytometric Analysis of BAFF Receptors

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Surface expression of BAFFR, BCMA, and TACI was analyzed by flow cytometry with PE-conjugated anti-TACI, anti-BCMA, or anti-BAFFR antibodies (Biolegend, San Diego, CA, USA) and compared to respective isotype-matched Ig controls. Cells were analyzed on Coulter FC-500 flow cytometer (Beckman-Coulter, Brea, CA, USA).
Sevdali E., Katsantoni E., Smulski C.R., Moschovi M., Palassopoulou M., Kolokotsa E.N., Argentou N., Giannakoulas N., Adamaki M., Vassilopoulos G., Polychronopoulou S., Germenis A.E., Eibel H, & Speletas M. (2019). BAFF/APRIL System Is Functional in B-Cell Acute Lymphoblastic Leukemia in a Disease Subtype Manner. Frontiers in Oncology, 9, 594.
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2

Characterization of Immune Cell Subsets in Blood and CSF

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Cryopreserved Ficoll density separated peripheral blood mononuclear cells were thawed by standard technique. Cells in the CSF were used within 3 hours of collection after isolation. CD3+/CD4+/CD8+ T cell, CD19+ B cell, and anti-BCMA directed T cells were measured by multiple-color flow cytometry with human monoclonal Acrobiosystems anti-BCMA (FITC) (cat# BCA-HF254-25ug) and BioLegend human monoclonal anti-CD3 (cat# 300472 and cat# 344842), human monoclonal anti-CD4 (cat# 317434), human monoclonal anti-CD8 (cat# 344742), human monoclonal anti-CD19 (cat# 561121). All cell surface antibodies were used at a 1:20 dilution following the manufacturer’s recommendation. The FITC-labeled human BCMA as used at a 1:100 dilution. The samples were acquired on a BD FACS LSR Fortessa flow cytometry system (BD Biosciences). Data was visualized and analyzed using Cytobank21 .
Van Oekelen O., Aleman A., Upadhyaya B., Schnakenberg S., Madduri D., Gavane S., Teruya-Feldstein J., Crary J.F., Fowkes M.E., Stacy C.B., Kim-Schulze S., Rahman A., Laganà A., Brody J.D., Merad M., Jagannath S, & Parekh S. (2021). Neurocognitive and hypokinetic movement disorder with features of parkinsonism following BCMA-targeting CAR-T cell therapy. Nature medicine, 27(12), 2099-2103.
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