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B6 cg tg thy1 yfp hjrs j mice

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B6.Cg-Tg(Thy1-YFP)HJrs/J mice are a genetically modified mouse strain that express yellow fluorescent protein (YFP) under the control of the Thy1 promoter. This results in the labeling of specific neuronal populations with YFP.

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6 protocols using b6 cg tg thy1 yfp hjrs j mice

1

Tissue Clearing Optimization for Thy1-YFP Mice

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For comparison of the clearing performance, brains of adult (4–5 months) B6.Cg-Tg(Thy1-YFP)HJrs/J mice (Jackson Laboratory) were used. The tissue was provided by the Department for Pharmacology and Toxicology of Maastricht University. The mice were bred under breeding license number B2015–003 and all brains provided were from mice from the breeding colonies, sacrificed as part of the breeding plan (surplus mice from breeding). Animals were sacrificed by CO2 inhalation in accordance with the EU guideline and the local animal regulations. Brains were collected directly and immersion fixated in 4% PFA in 0.1 M PBS at pH 7.4 for 24 h before being transferred to 0.1 M PBS containing 0.1% sodium azide. Brains were then cut into 3 mm thick coronal sections for clearing or processed without further dissection.
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2

Transgenic Mice for Ophthalmic Research

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B6.Cg-Tg(Thy1-YFP)HJrs/J mice (Jackson Laboratory, Bar Harbor, ME; Stock number: 003782) were used in each of four groups: control, ONT, and two EG groups. The mice were bred at the Carleton Animal Care Facility, Dalhousie University, Halifax, Canada. Protocols were approved by the Dalhousie University Committee on Laboratory Animals, and all procedures were performed in accordance with regulations established by the Canadian Council on Animal Care and the Association for Research in Vision and Ophthalmology Statement for the use of animals in Ophthalmic and Vision Research.
Mice were anesthetized with 2% isoflurane (Baxter Corporation, Mississauga, ON, Canada) and 1% O2 administered via a nose cone. Anesthesia was achieved with this method for all procedures.
A CSLO device modified for rodents11 (Spectralis Multiline, Heidelberg Engineering GmbH, Heidelberg, Germany) was used to screen mice from the breeding colony with YFP-positive RGCs for use in the experiments. The imaging procedure is described below.
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3

Detailed Murine Neuroscience Protocols

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All animal studies presented here were approved by the UCLA Animal Research Committee, accredited by the AAALAC. Mice were housed under UCLA regulation with a 12-h dark-light cycle. All mice used in the study were male. Wild-type C57Bl/6 mice (Jackson Labs, Strain #000667) were used for all experiments unless otherwise stated. Male YFP-H transgenic mice (derived from B6.Cg-Tg (Thy1-YFP)HJrs/J mice, Jackson Labs, Strain #003782) were used for tissue clearing studies.
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4

Thy1-YFP-H Mouse Model Study

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Adult female B6.Cg-Tg(Thy1-YFP)HJrs/J mice (referred to as Thy1-YFP-H, stock no. 003782; Jackson Laboratory, Bar Harbor, ME, USA) aged 11 to 12 months were used in this study. They were housed on a 12-hour light/dark cycle with standard rodent chow and water as desired. All animal operations were approved by the Institutional Animal Care and Use Committee of Zhongshan Ophthalmic Center and complied with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research.
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5

Thy1-YFP Transgenic Mouse Model

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Male B6.Cg-Tg(Thy1-YFP)HJrs/J mice (Jackson Laboratories, Bar Harbor, ME) between 8–10 weeks of age were used for all experiments. Mice were maintained in a 12 h dark/light cycle with access to food and water ad libitum. All procedures were performed in accordance to the guidelines of the National Institutes of Health and the Chancellor’s Animal Research Committee of the University of California, Los Angeles Office for the Protection of Research Subjects.
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6

Optogenetic Manipulation of vGAT Neurons

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The behavioral data were collected from 7 adult (P90-P365) male mice whose genotypes are either (1) C57BL/6J [n = 2], (2) Slc6a3tm1.1(cre)Bkmn/J [n = 2], or (3) B6.Cg-Tg(Slc32a1-COP4*H134R/EYFP)8Gfng/J [n = 3; called vGAT-ChR2 in this report]. The same set of vGAT-ChR2 mice were used for photoinhibition experiments. These mice were obtained from the Jackson Laboratory (see Resources Table). For the photo-bleaching assay (Figure S4), we used B6.Cg-Tg(Thy1-YFP)HJrs/J mice (n = 3, Jackson Laboratory). For the electrophysiological confirmation of inhibition (Figure S4), we used 5 additional vGAT-ChR2 mice. All mice were housed on a 12h dark/12h light cycle (dark from 06:00–18:00) and each performed the behavioral task at the same time of day, between 07:00 and 19:00. After surgery they were individually housed. All mice were maintained above 90% of their initial weight, and no mouse was given less than 1.5 mL water per day. All surgical and experimental procedures were in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and approved by the Harvard Institutional Animal Care and Use Committee. A subset of mice (n = 2) in this study was first tested in a similar adaptation task (unpublished data) prior to participating in this study (but in no more than 8 sessions).
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