Ge amersham imager ai680

Manufactured by GE Healthcare

Sourced in United States

The GE Amersham Imager AI680 is a compact and versatile imaging system designed for a range of life science applications. It utilizes a high-resolution camera and advanced imaging technology to capture high-quality images of gel-based assays, blots, and other samples.

Automatically generated - may contain errors

Lab products found in correlation

Amersham imager, by Cytiva (4 mentions) Ripa buffer, by Thermo Fisher Scientific (1 mentions) Pierce ecl western blotting substrate, by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by GoldBio (1 mentions) Bca protein assay kit, by Sangon (1 mentions) Ripa lysis buffer, by Boster Bio (1 mentions) Chemiluminescence reagent, by Beyotime (1 mentions) Nitrocellulose transfer membrane, by GE Healthcare (1 mentions) Bca protein assay kit, by Beyotime (1 mentions) Ipvh00010, by Merck Group (1 mentions) P7170, by Merck Group (1 mentions) Nupage bis tris sds page, by Thermo Fisher Scientific (1 mentions) Mops sds running buffer, by Merck Group (1 mentions) Immobilon crescendo western hrp substrate, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Amersham Imager (31 citations) Amersham Imager AI680 (9 citations) AI680 (7 citations) AI680 Imager (6 citations)

4 protocols using ge amersham imager ai680

Western Blot Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cells and bone tissues were lysed with RIPA buffer (Thermo Scientific). The total proteins were measured spectrophotometrically using Pierce Coomassie Plus (Bradford) Protein Assay kit (Thermo Scientific). Normalized protein samples were separated by SDS-PAGE electrophoresis and transferred to PVDF membranes for 2hrs in a cold transfer buffer. The membranes were blocked with 10 ml of PBST containing 5% BSA (GoldBio) for 30 min, incubated with primary antibodies (Table S8) at 4 °C overnight, secondary antibodies (Table S8) for 2hrs at room temperature and Pierce™ ECL Western Blotting Substrate (Thermo Scientific). The targeted protein band intensities were analyzed using GE Amersham Imager AI680.

Kubi J.A., Brah A.S., Cheung K.M., Lee Y.L., Lee K.F., Sze S.C., Qiao W, & Yeung K.W. (2023). A new osteogenic protein isolated from Dioscorea opposita Thunb accelerates bone defect healing through the mTOR signaling axis. Bioactive Materials, 27, 429-446.

+ Open protocol

+ Expand

Western Blot Analysis of U251 and U87 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

U251 and U87 cells were lysed using the RIPA Lysis Buffer (AR0105–100, Boster, Pleasanton, CA, USA) containing 1 mM phenylmethylsulfonyl fluoride (PMSF). After being centrifuged, the protein concentration was measured with a bicinchoninic acid (BCA) protein assay kit (C503021, Sangon Biotech). The proteins were electrophoresed on 12–15% SDS–PAGE gels and then transferred onto polyvinylidene fluoride (PVDF) membranes. After that, the membranes were incubated with 5% skimmed milk for 1 h at room temperature (RT), indicated primary antibodies overnight at 4 °C, and then with the appropriate secondary antibodies for 1 h at RT in turn. The blots were imaged with an enhanced chemiluminescence detection system and GE Amersham Imager AI680 (GE, Chicago, IL, USA). β-actin was used as a loading control. Data were analyzed by ImageJ software (US National Institutes of Health).

Yu J., Gao G., Wei X, & Wang Y. (2022). TTK Protein Kinase promotes temozolomide resistance through inducing autophagy in glioblastoma. BMC Cancer, 22, 786.

+ Open protocol

+ Expand

Western Blot Analysis of Protein Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells and tissues were lysed in radioimmunoprecipitation assay lysis buffer. Total protein was determined using a BCA Protein Assay Kit (Beyotime, Shanghai, China). Equivalent amounts of total protein were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to a nitrocellulose transfer membrane (GE Healthcare Life Sciences, Chicago, IL, USA). Primary antibodies were incubated overnight at 4°C after blocking with 5% nonfat milk for 2 hours. The membranes were then incubated with horseradish peroxidase–conjugated secondary antibodies for 2 hours at room temperature. Finally, the proteins were visualized with chemiluminescence reagents (Beyotime, China). The films were scanned using a GE Amersham Imager AI680 (GE Healthcare Life Sciences).

Peng F., Jiang D., Xu W., Sun Y., Zha Z., Tan X., Yu J., Pan C., Zheng Q, & Chen W. (2022). AMPK/MFF Activation: Role in Mitochondrial Fission and Mitophagy in Dry Eye. Investigative Ophthalmology & Visual Science, 63(12), 18.

+ Open protocol

+ Expand

Protein Denaturation and Western Blotting

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples were denatured in SDS sample buffer (6X) (0.375 M Tris pH 6.8, 12 % SDS, 60 % glycerol, 0.6 M DTT, 0.06 % bromophenol blue) for 5 min at 95 °C. 10–20 μg of protein was resolved on a 4–12% NuPAGE BisTris SDS–PAGE (Invitrogen) with MOPS SDS Running Buffer (Sigma Aldrich, M1254).
SDS-PAGE used for silver staining (Life Technologies, 24612) were processed according to the manufactures protocol, respectively.
For western blot, SDS-PAGE was transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore, IPVH00010) using Towbin transfer buffer (25 mM Tris, 192 mM glycine, 20% (v/v) methanol). To control for correct loading and transfer, Ponceau staining was performed according to manufactures instructions (Sigma Aldrich, P7170). Membranes were blocked in 5% BSA or 5% milk in TBS containing 0.05% Tween (TBST) and incubated overnight at 4 °C with primary antibody. Secondary HRP-conjugated antibodies were used, membranes were incubated in Immobilon Crescendo Western HRP substrate (Fisher Scientific, WBLUR0500), and imaged (GE Amersham Imager AI680). Used antibodies are displayed in the key resource table.

Mittenbühler M.J., Jedrychowski M.P., van Vranken J.G., Sprenger H.G., Wilensky S., Dumesic P.A., Sun Y., Tartaglia A., Bogoslavski D., A M., Xiao H., Blackmore K.A., Reddy A., Gygi S.P., Chouchani E.T, & Spiegelman B.M. (2023). Isolation of extracellular fluids reveals novel secreted bioactive proteins from muscle and fat tissues. Cell metabolism, 35(3), 535-549.e7.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!