Hnrnpc1 c2

Four fold of SDS-PAGE sample buffer (200 mM Tris pH 6.8, 8 % SDS, 0.4 % bromophenol blue, 40 % glycerol, 400 mM β-mercaptoethanol) was added to the sample to a final concentration of 1 fold and then heated at 100 °C for 5 min. Proteins were separated on 10 % polyacrylamide gels and transferred onto a 0.45 μm-pore-size polyvinylidene difluoride membrane (Millipore, Billerica, MA) for western blotting. The membrane was incubated for 1 h at room temperature with an antibody against any of the following proteins: BRF1/2, phosphorylated p38 (p-p38) MAPK T180/Y182, p-p44/42 MAPK (all from Cell Signaling), hnRNPC1/C2, MKP-1, ERK1, JNK1 (all from Santa Cruz Biotechnology), total-p38, p-JNK, Flag M2 (all from Sigma-Aldrich), Ttp, Zfp36l1, Zfp36l2, and β-tubulin [26 (link)]. After washing with PBST (PBS containing 0.1 % (v/v) Tween 20) for an appropriate time, the membrane was incubated for 1 h at room temperature with a horseradish peroxidase–conjugated secondary antibody: goat anti-rabbit IgG (KPL, Gaithersburg, ML), goat anti-mouse IgG (KPL), or rabbit anti-goat (Sigma-Aldrich). Western Lightning enhanced chemiluminescence substrate (Perkin Elmer, Norwalk, CT) was used for detection.

R-IgG (SC-2027), m-IgG (SC-2025), Actin (SC-47778), H3K9me3 (Cell Signaling, 9754), H3K4me3 (Cell Signaling, 9751; active motif 39159), H3K27me3 (Cell Signaling, 9733), H3K9ac (Cell Signaling, 9649), H3K36me (Cell Signaling, 4909), H3K27ac (ab4729), H3K9ac (ab176916), rabbit polyclonal Ki67 (Vectorlabs), MLL1 (Active motif, 61296), Lamin A/C (E-1), hnRNPC1/C2 (Santa Cruz, SC-32308), SAFA (Santa Cruz, SC-32315), U2AF65 (Santa Cruz, SC-53942), DDX3 (Santa Cruz, SC-365768), hnRNPA1 (Santa Cruz, SC-32301), hnRNPD (abcam, ab61193), DDX21 (Santa Cruz, SC-376953), DNA Damage antibody sample kit (Cell Signaling, 9947), Apoptosis Antibody sampler Kit (Cell signaling, 9915).