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On targetplus smart

Manufactured by Thermo Fisher Scientific
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ON-TARGETplus SMART is a gene silencing reagent system designed for efficient and targeted knockdown of gene expression. The core function of this product is to provide a tool for researchers to modulate gene expression levels in cell-based studies.

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Lab products found in correlation

Lipofectamine rnaimax, by Thermo Fisher Scientific (6 mentions) Hydroxyurea, by Merck Group (2 mentions) Aphidicolin, by AG Scientific (2 mentions)

3 protocols using on targetplus smart

1

HUES6 Cell siRNA Knockdown

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HUES6 cells were transfected using Lipofectamine RNAiMAX (ThermoFisher) transfection reagent according to the manufacturer’s recommendations. ON-TARGETplus SMART pool of four siRNAs from Dharmacon (ThermoFisher) were used for siRNA knockdown: TRF1 (catalogue #L-010542-02-0005); BLM: (catalogue #L-007287-00-0005); WRN: (catalogue #L-010378-00-0005); Exo1: (catalogue #L-013120-00-0005); DNA2: (catalogue #L-026431-01-0005); SMARCAL1: (catalogue # L-013058-00-0005); XRCC3: (catalogue # L-012067-00-0005) or control non-targeting pool (catalogue # D-001810-10-20). The cells were collected 72 h or 96 h post treatment after two serial transfections and processed for protein, RNA and DNA analyses.
Cells were treated with 0.2mM or 3mM hydroxyurea (Sigma), 5μM aphidicolin (AG Scientific) or 0.5μM RHPS4 for 24h, and collected for DNA analysis.
Rivera T., Haggblom C., Cosconati S, & Karlseder J. (2016). A balance between elongation and trimming regulates telomere stability in stem cells. Nature structural & molecular biology, 24(1), 30-39.
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2

Silencing FGFR1/2 Impacts Rickettsia Infection

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ON-TARGETplus smart small-interfering RNA (siRNA) pools for FGFR1 and FGFR2 along with a standard negative siRNA control (Thermo Fisher) were transfected into ECs using Lipofectamine RNAimax™ according to the manufacturer's recommendations. Seventy two hours later, cells were infected with R. rickettsii and subjected to the isolation of DNA for subsequent determination of rickettsial copy number.
Sahni A., Patel J., Narra H.P., Schroeder C.L., Walker D.H, & Sahni S.K. (2017). Fibroblast growth factor receptor-1 mediates internalization of pathogenic spotted fever rickettsiae into host endothelium. PLoS ONE, 12(8), e0183181.
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3

HUES6 Cell siRNA Knockdown

Check if the same lab product or an alternative is used in the 5 most similar protocols
HUES6 cells were transfected using Lipofectamine RNAiMAX (ThermoFisher) transfection reagent according to the manufacturer’s recommendations. ON-TARGETplus SMART pool of four siRNAs from Dharmacon (ThermoFisher) were used for siRNA knockdown: TRF1 (catalogue #L-010542-02-0005); BLM: (catalogue #L-007287-00-0005); WRN: (catalogue #L-010378-00-0005); Exo1: (catalogue #L-013120-00-0005); DNA2: (catalogue #L-026431-01-0005); SMARCAL1: (catalogue # L-013058-00-0005); XRCC3: (catalogue # L-012067-00-0005) or control non-targeting pool (catalogue # D-001810-10-20). The cells were collected 72 h or 96 h post treatment after two serial transfections and processed for protein, RNA and DNA analyses.
Cells were treated with 0.2mM or 3mM hydroxyurea (Sigma), 5μM aphidicolin (AG Scientific) or 0.5μM RHPS4 for 24h, and collected for DNA analysis.
Rivera T., Haggblom C., Cosconati S, & Karlseder J. (2016). A balance between elongation and trimming regulates telomere stability in stem cells. Nature structural & molecular biology, 24(1), 30-39.
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